Li Yangyi, Huang Xingxue, Zhou Guolin, Ye Anhua, Deng Yaohua, Zhu Mengting, Wang Haixiao, Zhang Runhua
Institute of Vegetable Research, Wuhan Academy of Agricultural Sciences, Wuhan, 430045, Hubei, China.
Arch Virol. 2025 Jul 1;170(8):171. doi: 10.1007/s00705-025-06332-3.
A new negative-stranded ophio-like RNA mycovirus, Rhizoctonia solani mycoophiovirus 1 (RsMOPV1), was isolated from strain JZ56 of Rhizoctonia solani AG-1 IA. The full-length RNA genome of RsMOPV1 is 7,317 nucleotides (nt) in length and consists of a single long open reading frame (ORF). The ORF encodes an RNA-dependent RNA polymerase (RdRp) consisting of 7,138 amino acids, with a predicted molecular mass of 273 kDa. The coding region of the RsMOPV1 genome is flanked by a short structured 3' untranslated region (3'-UTR) of 58 nucleotides and a 5' untranslated region (5'-UTR) of 121 nucleotides. We did not detect any additional segments of RsMOPV1. The nucleotide sequence of the genome of RsMOPV1 shares 74.7% identity with that of Rhizoctonia solani negative-stranded virus 2. RsMOPV1 infections may be associated with hypovirulence and a slow growth rate in R. solani. The RdRp of RsMOPV1 contains five typical RdRp motifs. Phylogenetic analysis demonstrated that RsMOPV1 clusters with members of the previously proposed genus "Mycoophiovirus".
一种新的负链蛇形样RNA真菌病毒，立枯丝核菌真菌蛇形病毒1（RsMOPV1），从立枯丝核菌AG-1 IA菌株JZ56中分离得到。RsMOPV1的全长RNA基因组长度为7317个核苷酸（nt），由一个单一的长开放阅读框（ORF）组成。该ORF编码一个由7138个氨基酸组成的RNA依赖的RNA聚合酶（RdRp），预测分子量为273 kDa。RsMOPV1基因组的编码区两侧分别是一个58个核苷酸的短结构3'非翻译区（3'-UTR）和一个121个核苷酸的5'非翻译区（5'-UTR）。我们未检测到RsMOPV1的任何其他片段。RsMOPV1基因组的核苷酸序列与立枯丝核菌负链病毒2的核苷酸序列具有74.7%的同一性。RsMOPV1感染可能与立枯丝核菌的低毒力和生长速率缓慢有关。RsMOPV1的RdRp包含五个典型的RdRp基序。系统发育分析表明，RsMOPV1与先前提出的“真菌蛇形病毒属”成员聚类。
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