Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Shandong Provincial Key laboratory of Glycoscience and Glycoengineering, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Shandong Provincial Key laboratory of Glycoscience and Glycoengineering, Qingdao 266003, China.
Carbohydr Polym. 2021 Oct 1;269:118258. doi: 10.1016/j.carbpol.2021.118258. Epub 2021 Jun 2.
Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties. However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed. Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA). Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm. Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA. AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing. Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
海洋多糖或寡糖由于其生物相容性和物理化学性质，具有促进伤口愈合的潜力。然而，微生物感染会延迟伤口愈合过程，因此迫切需要新型抗菌伤口敷料。在这里，从海洋红藻中提取的琼脂寡糖 (AGO) 被用作绿色合成银纳米粒子 (AgNPs) 的还原剂和稳定剂，并进一步成功地与抗菌肽 (AMP) 之一的odorranain A (OA) 连接，得到一种新型复合纳米材料 (AGO-AgNPs-OA)。透射电子显微镜 (TEM) 和马尔文粒度分析仪显示，AGO-AgNPs-OA 呈球形或椭圆形，平均粒径约为 100nm。圆二色性 (CD) 光谱表明 AGO-AgNPs 稳定了 OA 的α-螺旋结构。与 AGO-AgNPs 相比，AGO-AgNPs-OA 具有更强的抗菌活性，并且具有良好的生物相容性和对伤口愈合的显著促进作用。我们的数据表明，与海洋寡糖和 AgNPs 结合的 AMP 可能是用于伤口治疗的有效且安全的抗菌材料。
