Firouzeh Nima, Asadi Arash, Tavakoli Kareshk Amir
Department of Parasitology and Mycology, School of Medicine, Kerman University of Medical Science, Kerman, Iran.
Infectious Disease Research Center, Birjand University of Medical Sciences, Birjand, Iran.
J Parasit Dis. 2021 Jun;45(2):366-371. doi: 10.1007/s12639-020-01308-3. Epub 2020 Nov 13.
Protozoan parasites, such as (), remained as a global health problem of the current century. is a major cause of cutaneous leishmaniasis (CL) in developed and developing countries. Traditionally, amphotericin B is prescribed as an alternative drug, while first-line drugs failed. Some active proteins of the innate immune system such as cathelicidins try to inhibit infection Via several proposed mechanisms. Here this research aimed to not only determine the anti-leishmanial activity of nano amphotericin B but also to evaluate which anti-leishmanial drug can induce the cathelicidin gene expression. Both promastigote and amastigote stages of were exposed to various concentrations of nano amphotericin B, amphotericin B and finally compared to glucan time as standard drug for CL treatment. For the gene expression of cathelicidin, macrophages were exposed to the same concentration of anti-leishmanial drugs. The findings demonstrated that nano amphotericin B was more effective at all concentrations than amphotericin B. Additionally, among tested anti-leishmanial drugs, nano amphotericin B has more potency to induce the cathelicidin gene expression in macrophages cells. The findings revealed that nano amphotericin B has potential as an effective anti-leishmanial drug against CL caused by parasites.
原生动物寄生虫，如（），仍是本世纪的一个全球健康问题。（）是发达国家和发展中国家皮肤利什曼病（CL）的主要病因。传统上，当一线药物无效时，两性霉素B被用作替代药物。一些先天免疫系统的活性蛋白，如抗菌肽，试图通过几种提出的机制抑制感染。本研究旨在不仅确定纳米两性霉素B的抗利什曼活性，还评估哪种抗利什曼药物能诱导抗菌肽基因表达。将（）的前鞭毛体和无鞭毛体阶段暴露于不同浓度的纳米两性霉素B、两性霉素B，并最终与作为CL治疗标准药物的葡聚糖时间进行比较。对于抗菌肽的基因表达，巨噬细胞暴露于相同浓度的抗利什曼药物。研究结果表明，在所有浓度下，纳米两性霉素B都比两性霉素B更有效。此外，在测试的抗利什曼药物中，纳米两性霉素B在巨噬细胞中诱导抗菌肽基因表达的能力更强。研究结果表明，纳米两性霉素B有潜力作为一种有效的抗利什曼药物，对抗由（）寄生虫引起的CL。
