Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Horticulture, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2022 Jun 21;23(13):6906. doi: 10.3390/ijms23136906.
Nuclear factor YB (NF-YB) are plant-specific transcription factors that play a critical regulatory role in plant growth and development as well as in plant resistance against various stresses. In this study, a total of 49 genes were identified from the genomes of and . Multiple sequence alignment analysis showed that all of these NF-YB members contain DNA binding domain, NF-YA interaction domain and NF-YC interaction domain. Phylogenetic analysis suggested that these NF-YB proteins could be classified into five distinct clusters. We also analyzed the exon-intron organizations and conserved motifs of these genes and their deduced proteins. We also found many stress-related -acting elements in their promoter region. In addition, analyses on genechip for and transcriptome data for indicated that these genes exhibited a distinct expression pattern in various tissues; many of these could be induced by drought and/or salt treatments. In particular, RT-qPCR analysis revealed that the expression levels of gene pairs and were significantly up-regulated under NaCl and mannitol treatments, indicating that they are most likely involved in salt and drought stress response. Taken together, our study on family genes in is valuable for their functional characterization, as well as for the application of genes in genetic breeding for high-yield and high-resistance alfalfa.
核因子 YB（NF-YB）是植物特异性转录因子，在植物生长发育以及植物对各种胁迫的抗性中发挥着关键的调控作用。本研究从 和 基因组中鉴定出了 49 个基因。多重序列比对分析表明，所有这些 NF-YB 成员都含有 DNA 结合域、NF-YA 相互作用域和 NF-YC 相互作用域。系统发育分析表明，这些 NF-YB 蛋白可以分为五个不同的簇。我们还分析了这些 基因及其推导蛋白的内含子-外显子组织和保守基序。我们还在启动子区域发现了许多与应激相关的顺式作用元件。此外，对 和 基因芯片的分析以及 转录组数据的分析表明，这些 基因在各种组织中表现出不同的表达模式；其中许多基因可以被干旱和/或盐处理诱导。特别是，RT-qPCR 分析表明，基因对 和 在 NaCl 和甘露醇处理下的表达水平显著上调，表明它们可能参与盐和干旱胁迫反应。总之，我们对 中 NF-YB 家族基因的研究对于它们的功能表征以及在遗传育种中应用 基因提高苜蓿的产量和抗性具有重要价值。
