Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawai'i at Hilo, Hilo, HI 96720, USA.
Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen 518172, China.
Molecules. 2021 Jul 12;26(14):4239. doi: 10.3390/molecules26144239.
is one of the most diverse genera, and it is chemically profound and known to produce many biologically active secondary metabolites. In the present study, a new aspochalasin H1 (), together with nine known compounds (-), were isolated from a Hawaiian plant-associated endophytic fungus sp. FT1307. The structures were elucidated using nuclear magnetic resonance (NMR) (H, H-H COSY, HSQC, HMBC, ROESY and 1D NOE), high-resolution electrospray ionization mass spectroscopy (HRESIMS), and comparisons with the reported literature. The absolute configuration of the new compound was established by electronic circular dichroism (ECD) in combination with NMR calculations. The new compound contains an epoxide moiety and an adjacent -diol, which has not been reported before in the aspochalasin family. The antibacterial screening of the isolated compounds was carried out against pathogenic bacteria (, Methicillin-resistant and ). The antiproliferative activity of compounds - was evaluated against human breast cancer cell lines (MCF-7 and T46D) and ovarian cancer cell lines (A2780).
是最具多样性的属之一，其化学结构复杂，已知能产生许多具有生物活性的次级代谢产物。在本研究中，从夏威夷植物内生真菌 sp. FT1307 中分离得到一种新的 Asperchalasin H1 ()，并与 9 种已知化合物 (-) 一起进行了分离。利用核磁共振波谱（NMR）（H、H-H COSY、HSQC、HMBC、ROESY 和 1D NOE）、高分辨电喷雾电离质谱（HRESIMS）和与文献报道的比较，确定了结构。通过电子圆二色性（ECD）与 NMR 计算相结合，确定了新化合物的绝对构型。新化合物含有一个环氧化物部分和一个相邻的 -二醇，这在 Asperchalasin 家族中以前没有报道过。对分离得到的化合物进行了针对致病菌（、耐甲氧西林金黄色葡萄球菌和 ）的抗菌筛选。对化合物 - 的抗增殖活性进行了评估，包括人乳腺癌细胞系（MCF-7 和 T46D）和卵巢癌细胞系（A2780）。
