DesRochers Natasha, Walsh Jacob P, Renaud Justin B, Seifert Keith A, Yeung Ken K-C, Sumarah Mark W
London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., London, ON N5V 4T3, Canada.
Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, ON N6A 3K7, Canada.
Metabolites. 2020 Jan 14;10(1):35. doi: 10.3390/metabo10010035.
Ginseng root is an economically valuable crop in Canada at high risk of yield loss caused by the pathogenic fungus formerly known as . While this pathogen has been well-characterized from morphological and genetic perspectives, little is known about the secondary metabolites it produces and their role in pathogenicity. We used an untargeted tandem liquid chromatography-mass spectrometry (LC-MS)-based approach paired with global natural products social molecular networking (GNPS) to compare the metabolite profiles of virulent and avirulent strains. The ethyl acetate extracts of 22 strains and closely related species were analyzed by LC-MS/MS. Principal component analysis of LC-MS features resulted in two distinct groups, which corresponded to virulent and avirulent strains. Virulent strains produced more types of compounds than the avirulent strains. The previously reported antifungal compound radicicol was present. Additionally, a number of related resorcyclic acid lactones (RALs) were putatively identified, namely pochonins and several additional derivatives of radicicol. Pochonins have not been previously reported in spp. and have documented antimicrobial activity. This research contributes to our understanding of natural products and its pathogenic relationship with ginseng.
人参根在加拿大是一种具有经济价值的作物，极易因以前称为 的致病真菌而导致产量损失。虽然这种病原体已从形态学和遗传学角度得到充分表征，但对其产生的次生代谢产物及其在致病性中的作用却知之甚少。我们采用基于非靶向串联液相色谱 - 质谱（LC-MS）的方法，并结合全球天然产物社会分子网络（GNPS）来比较有毒和无毒 菌株的代谢物谱。通过LC-MS/MS分析了22株 菌株和密切相关物种的乙酸乙酯提取物。对LC-MS特征进行主成分分析产生了两个不同的组，分别对应于有毒和无毒 菌株。有毒菌株产生的化合物类型比无毒菌株更多。之前报道的抗真菌化合物radicicol存在。此外，还推定鉴定出了一些相关的间苯二酚酸内酯（RALs），即pochonins和radicicol的几种其他衍生物。Pochonins以前在 物种中尚未见报道，并且具有已记录的抗菌活性。这项研究有助于我们了解 的天然产物及其与人参的致病关系。
