Shenyang Pharmaceutical University, Shenyang, PR China.
Fitoterapia. 2012 Jul;83(5):913-20. doi: 10.1016/j.fitote.2012.04.009. Epub 2012 Apr 16.
To screen the presence of ginkgolide B-producing endophytic fungi from the root bark of Ginkgo biloba, a total of 27 fungal isolates, belonging to 6 different genus, were isolated from the internal root bark of the plant Ginkgo biloba. The fungal isolates were fermented on solid media and their metabolites were analyzed by TLC. The obtained potential ginkgolides-producing fungus, the isolate SYP0056 which was identified as Fusarium oxysporum, was successively cultured in the liquid fermentation media, and its metabolite was analyzed by HPLC. The ginkgolide B was successfully isolated from the metabolite and identified by HPLC/ESI-MS and (13)C-NMR. The current research provides a new method to produce ginkgolide B by fungal fermentation, which could overcome the natural resource limitation of isolating from the leaves and barks of the plant Ginkgo biloba.
为了从银杏的根皮中筛选出产生银杏内酯 B 的内生真菌，从银杏植物的内部根皮中分离出了 27 株真菌分离株，属于 6 个不同属。将真菌分离株在固体培养基上发酵，并通过 TLC 分析其代谢产物。从获得的具有潜在产生银杏内酯能力的真菌，即被鉴定为尖孢镰刀菌的分离株 SYP0056，在液体发酵培养基中进行连续培养，并通过 HPLC 分析其代谢产物。银杏内酯 B 从代谢产物中成功分离出来，并通过 HPLC/ESI-MS 和 (13)C-NMR 进行了鉴定。本研究为通过真菌发酵生产银杏内酯 B 提供了一种新方法，可克服从银杏植物的叶和树皮中分离的天然资源限制。
