Yamamura T, Takahashi T, Kusunoki M, Ishikawa Y, Kantoh M, Utsunomiya J
Second Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan.
Jpn J Surg. 1987 Sep;17(5):388-94. doi: 10.1007/BF02470639.
We studied the effects of progesterone on the contractile motility of the guinea pig gallbladder in vitro. Carbachol (10(-6) M) induced contractions were reduced by the pretreatment with progesterone (10(-8)-10(-6) M) in a dose-dependent manner. Concentration-response curves for carbachol, histamine and CCK-OP showed inhibition by progesterone (5 X 10(-7) M). These results suggest that progesterone has a direct inhibitory effect on gallbladder smooth muscle. Contractile responses to potassium (10-60 mM) or calcium (0.4-3.2 mM), which were thought to activate the contractile machinery by increasing the influx of extracellular calcium, were not affected by the pretreatment of progesterone. The direct inhibitory effects of progesterone on gallbladder smooth muscle might be explained by the inhibition of calcium release from the intracellular storage sites.
我们在体外研究了孕酮对豚鼠胆囊收缩运动的影响。用孕酮（10⁻⁸ - 10⁻⁶ M）预处理后，卡巴胆碱（10⁻⁶ M）诱导的收缩呈剂量依赖性降低。卡巴胆碱、组胺和胆囊收缩素八肽的浓度 - 反应曲线显示，孕酮（5×10⁻⁷ M）具有抑制作用。这些结果表明，孕酮对胆囊平滑肌有直接抑制作用。对钾（10 - 60 mM）或钙（0.4 - 3.2 mM）的收缩反应，被认为是通过增加细胞外钙内流来激活收缩机制，但不受孕酮预处理的影响。孕酮对胆囊平滑肌的直接抑制作用可能是由于抑制了细胞内储存部位的钙释放。
