增生性玻璃体视网膜病变中视网膜前组织的蛋白质组。
The Proteome of Preretinal Tissue in Proliferative Vitreoretinopathy.
出版信息
Ophthalmic Surg Lasers Imaging Retina. 2021 Jul;52(S1):S5-S12. doi: 10.3928/23258160-20210518-02. Epub 2021 Jul 1.
BACKGROUND AND OBJECTIVE
Proliferative vitreoretinopathy (PVR) is the leading cause of retinal detachment repair failure. However, the molecular pathogenesis remains incompletely understood. Determining the proteome of PVR will help to identify novel therapeutic targets.
MATERIALS AND METHODS
Preretinal tissue samples, delaminated during surgery from six PVR cases and one idiopathic epiretinal membrane (ERM) were analyzed by mass spectrometry. Tandem mass spectra were extracted using the UniProt database, generating a list of 896 proteins, which were subjected to pathway set and fold-change (ERM vs PVR) analyses.
RESULTS
Two pathways were enriched in PVR: extracellular matrix (ECM) organization and extracellular structure organization. A fold-change analysis comparing mean total spectral counts from PVR to an ERM control identified fibronectin, the ECM glycoprotein, as the protein most significantly elevated in PVR compared to ERM.
CONCLUSION
These data identify pathwayskey to PVR progression, including thoseinvolved in cell-mediated ECM assembly and thus tractional force generation at the cellular level. .
背景与目的
增生性玻璃体视网膜病变（PVR）是视网膜脱离修复失败的主要原因。然而，其分子发病机制仍不完全清楚。确定 PVR 的蛋白质组将有助于确定新的治疗靶点。
材料与方法
通过质谱分析从 6 例 PVR 病例和 1 例特发性视网膜前膜（ERM）手术中分离的术前视网膜组织样本。使用 UniProt 数据库提取串联质谱，生成了 896 种蛋白质的列表，对其进行了途径集和倍数变化（ERM 与 PVR）分析。
结果
有两条途径在 PVR 中富集：细胞外基质（ECM）组织和细胞外结构组织。将 PVR 与 ERM 对照的平均总光谱计数的倍数变化进行比较，确定纤维连接蛋白，即 ECM 糖蛋白，在 PVR 中与 ERM 相比显著升高。
结论
这些数据确定了 PVR 进展的关键途径，包括参与细胞介导的 ECM 组装的途径，从而在细胞水平上产生牵引力。
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