Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004920.
In the present study, four bacterial strains, two (S-713 and 406) isolated from faecal samples of Tibetan antelopes and the other two (S-531 and 1598) from leaves of dandelion collected on the Qinghai-Tibet Plateau of PR China, were analysed using a polyphasic approach. All four isolates were aerobic, rod-shaped, non-motile, oxidase-negative, Gram-stain-positive and catalase-positive. According to four phylogenetic trees, strain pairs S-713/406 and S-531/1598 form two independent branches belonging to the genus , and are closest to , , , , and . Although sharing MK8-(H) as their major isoprenoid quinone, strains S-713 and S-531 contained C 9 (24.64 and 16.34 %) and iso-C (9.74 and 29.38 %), respectively, as their main fatty acids, with remarkable differences in their biochemical profiles but only slight ones in their optimal growth conditions. The chromosomes of strains S-713 and S-531 were 4 207 844 bp (G+C content, 73.0 mol%) and 4 809 817 bp (G+C content, 72.5 mol%), respectively. Collectively, the two strain pairs represent two separate novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, with S-713 (=JCM 33698=CGMCC 4.7660) and S-531 (=JCM 33468=CGMCC 4.7659) as the respective type strains.
在本研究中,我们采用多相分析的方法对从青藏高原采集的藏羚羊粪便样本中分离得到的两株(S-713 和 406)和蒲公英叶片中分离得到的另外两株(S-531 和 1598)细菌菌株进行了分析。这四株分离株均为需氧、杆状、非运动性、氧化酶阴性、革兰氏阳性和过氧化氢酶阳性。根据四个系统发育树,菌株对 S-713/406 和 S-531/1598 形成两个独立的分支,属于 属,与 、 、 、 、 最为接近。尽管 S-713 和 S-531 菌株的主要类异戊二烯醌均为 MK8-(H),但它们的主要脂肪酸分别为 C 9 (24.64%和 16.34%)和 iso-C(9.74%和 29.38%),生化特征差异显著,但最适生长条件仅略有不同。S-713 和 S-531 菌株的染色体分别为 4 207 844 bp(G+C 含量为 73.0%mol%)和 4 809 817 bp(G+C 含量为 72.5%mol%)。综上所述,这两个菌株对代表了 属的两个新种,分别命名为 sp. nov. 和 sp. nov.,其中 S-713(=JCM 33698=CGMCC 4.7660)和 S-531(=JCM 33468=CGMCC 4.7659)分别为它们的模式菌株。