Baldassarri A M, Giomini M, Giuliani A M, Trotta E, Boicelli C A
CNR Istituto San Raffaele, Milano, Italy.
Physiol Chem Phys Med NMR. 1987;19(2):95-100.
The metabolic changes occurring during liver regeneration after partial hepatectomy have been followed by examination of the perchloric extracts by means of NMR spectroscopy. Proton spectra show an abrupt decrease of glycogen, glucose and nucleotides, which are essentially ribonucleotides, in the first hours after hepatectomy. Nucleotides begin to store up again in the third day after hepatectomy, while glucose and glycogen storage builds up from the second day. 31P data evidence a sharp drop of phosphomonoesters, i.e. monophosphosugars (including AMP) and phosphocholine, and phosphodiesters, i.e. GPC and GPE, soon after hepatectomy and a recovery of the initial levels approximately at the 60th hour and a further increase of PDE at later times. The NMR findings are in agreement with the biochemical knowledge of the course of liver regeneration.
通过核磁共振光谱法对高氯酸提取物进行检测，追踪了部分肝切除术后肝脏再生过程中的代谢变化。质子光谱显示，肝切除术后最初几个小时内，糖原、葡萄糖和核苷酸（主要是核糖核苷酸）急剧减少。核苷酸在肝切除术后第三天开始再次蓄积，而葡萄糖和糖原的蓄积从第二天开始。31P数据表明，肝切除术后不久，磷酸单酯（即单磷酸糖，包括AMP和磷酸胆碱）和磷酸二酯（即甘油磷酸胆碱和甘油磷酸乙醇胺）急剧下降，大约在第60小时恢复到初始水平，之后磷酸二酯进一步增加。核磁共振研究结果与肝脏再生过程的生化知识相符。
