Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech Republic; Footwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou IV, 3685 Zlin, Czech Republic.
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech Republic; Footwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou IV, 3685 Zlin, Czech Republic.
Int J Pharm. 2021 Sep 25;607:120952. doi: 10.1016/j.ijpharm.2021.120952. Epub 2021 Jul 27.
In this study, an antimicrobial mumio-based hydrogel dressing was developed for wound healing application. The mechanism of gel formation was achieved via a double crosslink network formation between gelatin (GT) and polyvinyl alcohol (PVA) using polyethylene glycol diglycidyl ether (PEGDE) and borax as crosslinking agents. To enhance the mechanical integrity of the hydrogel matrix, bacterial cellulose (BC) was integrated into the GT-PVA hydrogel to produce a composite gel dressing. The obtained hydrogel was characterized by FTIR, SEM, TGA, and XRD. Gel fraction, in vitro swelling and degradation as well as compressive modulus properties of the gel dressing were investigated as a function of change in PVA and BC ratios. By increasing the ratios of PVA and BC, the composite dressing showed lower swelling but higher mechanical strength. Comparing to other formulations, the gel with 4 %w/v PVA and 1 %w/v BC demonstrated to be most suitable in terms of stability and mechanical properties. In vitro cell cytotoxicity by MTT assay on human alveolar basal epithelial (A549) cell lines validated the gels as non-toxic. In addition, the mumio-based gel was compared to other formulations containing different bioactive agents of beeswax and cinnamon oil, which were tested for microbial growth inhibition effects against different bacteria (S. aureus and K. pneumoniae) and fungi (C. albicans and A. niger) strains. Results suggested that the gel dressing containing combinations of mumio, beeswax and cinnamon oil possess promising future in the inhibition of microbial infection supporting its application as a suitable dressing for wound healing.
在这项研究中，开发了一种基于抗菌马尿酸的水凝胶敷料，用于伤口愈合应用。凝胶形成的机制是通过使用聚乙二醇二缩水甘油醚（PEGDE）和硼砂作为交联剂，在明胶（GT）和聚乙烯醇（PVA）之间形成双交联网络来实现的。为了增强水凝胶基质的机械完整性，将细菌纤维素（BC）整合到 GT-PVA 水凝胶中，以生产复合凝胶敷料。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、热重分析（TGA）和 X 射线衍射（XRD）对获得的水凝胶进行了表征。研究了凝胶分数、体外溶胀和降解以及凝胶敷料的压缩模量性能随 PVA 和 BC 比例变化的关系。通过增加 PVA 和 BC 的比例，复合敷料表现出较低的溶胀率，但较高的机械强度。与其他配方相比，含 4%w/v PVA 和 1%w/v BC 的凝胶在稳定性和机械性能方面表现出最佳效果。通过 MTT 法对人肺泡基底上皮（A549）细胞系进行体外细胞毒性评估，验证了这些凝胶无毒性。此外，将马尿酸基凝胶与其他含有不同生物活性剂（蜂蜡和肉桂油）的配方进行了比较，这些配方测试了对不同细菌（金黄色葡萄球菌和肺炎克雷伯菌）和真菌（白色念珠菌和黑曲霉）菌株的微生物生长抑制效果。结果表明，含有马尿酸、蜂蜡和肉桂油组合的凝胶敷料在抑制微生物感染方面具有广阔的应用前景，支持其作为一种合适的伤口愈合敷料的应用。
