Alonso Vanessa Pereira Perez, Harada Andréia Miho Morishita, Kabuki Dirce Yorika
Department of Food Science, Faculty of Food Engineering, University of Campinas, Campinas, Brazil.
Front Microbiol. 2020 Feb 28;11:177. doi: 10.3389/fmicb.2020.00177. eCollection 2020.
Microorganisms in dairy industries can form monospecies, dual-species, or multispecies biofilms, showing cooperative or competitive behaviors, which might contribute to the reduction of efficiency of cleaning and sanitization processes and eventually turn into a potential source of contamination. This study proposes to evaluate the behavior of in monospecies biofilms, cocultured with . The isolates were of dairy origin, and the selection occurred after studies of competition among species. The biofilm formations on AISI 304 stainless steel at 25°C in a stationary culture were analyzed to observe the cooperative or competitive interactions among species, as well as the effect of pre-adhered cells. Biofilm formation assays were performed in four experiments: Experiment 1: in the presence of strains of antagonistic substance producer (+); Experiment 2: extract of the antagonistic substance of ; Experiment 3: pre-adhered cells of ; and Experiment 4: pre-adhered cells of . Subsequently, cooperative behavior was observed by scanning electron microscopy. The monospecies biofilm counts of greater than 5 log colony-forming units (CFU)/cm were also observed in dual-species biofilms in the presence of (non-producers of antagonist substance), showing cooperative behavior between species. However, in the presence of antagonistic substance produced by , the counts were lower, 1.39 and 1.70 log CFU/cm ( > 0.05), indicating that the antagonistic substance contributes to competitive interactions. These data are relevant for the development of new studies to control in the dairy industry.
乳制品行业中的微生物可形成单物种、双物种或多物种生物膜，表现出合作或竞争行为，这可能会降低清洁和消毒过程的效率，并最终成为潜在的污染源。本研究旨在评估 [未提及具体微生物名称 1] 在与 [未提及具体微生物名称 2] 共培养的单物种生物膜中的行为。这些分离株源自乳制品，是在对物种间竞争进行研究后挑选出来的。分析了在 25°C 静止培养条件下 AISI 304 不锈钢上的生物膜形成情况，以观察物种间的合作或竞争相互作用，以及预先附着细胞的影响。生物膜形成试验在四个实验中进行：实验 1：在拮抗物质产生菌株 [未提及具体微生物名称 1]（+）存在的情况下；实验 2：[未提及具体微生物名称 2] 的拮抗物质提取物；实验 3：[未提及具体微生物名称 1] 的预先附着细胞；实验 4：[未提及具体微生物名称 2] 的预先附着细胞。随后，通过扫描电子显微镜观察合作行为。在存在 [未提及具体微生物名称 3]（非拮抗物质产生者）的双物种生物膜中，也观察到 [未提及具体微生物名称 1] 的单物种生物膜计数大于 5 log 菌落形成单位（CFU）/cm，表明物种间存在合作行为。然而，在存在 [未提及具体微生物名称 2] 产生的拮抗物质时，计数较低，为 1.39 和 1.70 log CFU/cm（P>0.05），表明拮抗物质促成了竞争相互作用。这些数据对于开展控制乳制品行业中 [未提及具体微生物名称 1] 的新研究具有重要意义。
