Chamarthi Siva K, Kaler Avjinder S, Abdel-Haleem Hussein, Fritschi Felix B, Gillman Jason D, Ray Jeffery D, Smith James R, Dhanapal Arun P, King Charles A, Purcell Larry C
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR, United States.
USDA-ARS, U.S. Arid Land Agricultural Research Center, Maricopa, AZ, United States.
Front Plant Sci. 2021 Jul 14;12:698116. doi: 10.3389/fpls.2021.698116. eCollection 2021.
Drought causes significant soybean [ (L.) Merr.] yield losses each year in rain-fed production systems of many regions. Genetic improvement of soybean for drought tolerance is a cost-effective approach to stabilize yield under rain-fed management. The objectives of this study were to confirm previously reported soybean loci and to identify novel loci associated with canopy wilting (CW) using a panel of 200 diverse maturity group (MG) IV accessions. These 200 accessions along with six checks were planted at six site-years using an augmented incomplete block design with three replications under irrigated and rain-fed treatments. Association mapping, using 34,680 single nucleotide polymorphisms (SNPs), identified 188 significant SNPs associated with CW that likely tagged 152 loci. This includes 87 SNPs coincident with previous studies that likely tagged 68 loci and 101 novel SNPs that likely tagged 84 loci. We also determined the ability of genomic estimated breeding values (GEBVs) from previous research studies to predict CW in different genotypes and environments. A positive relationship ( ≤ 0.05;0.37 ≤ r ≤ 0.5) was found between observed CW and GEBVs. In the vicinity of 188 significant SNPs, 183 candidate genes were identified for both coincident SNPs and novel SNPs. Among these 183 candidate genes, 57 SNPs were present within genes coding for proteins with biological functions involved in plant stress responses. These genes may be directly or indirectly associated with transpiration or water conservation. The confirmed genomic regions may be an important resource for pyramiding favorable alleles and, as candidates for genomic selection, enhancing soybean drought tolerance.
在许多地区的雨养生产系统中，干旱每年都会导致大豆[（L.）Merr.]产量大幅损失。通过遗传改良提高大豆的耐旱性是在雨养管理条件下稳定产量的一种经济有效的方法。本研究的目的是确认先前报道的大豆基因座，并使用一组200个不同的IV成熟组种质鉴定与冠层萎蔫（CW）相关的新基因座。这200个种质与6个对照品种一起，采用增广不完全区组设计，在灌溉和雨养处理下进行三次重复，在6个地点年份种植。利用34680个单核苷酸多态性（SNP）进行关联分析，鉴定出188个与CW显著相关的SNP，这些SNP可能标记了152个基因座。其中包括87个与先前研究一致的SNP，可能标记了68个基因座，以及101个新的SNP，可能标记了84个基因座。我们还确定了先前研究中的基因组估计育种值（GEBV）预测不同基因型和环境中CW的能力。观察到的CW与GEBV之间存在正相关关系（≤0.05；0.37≤r≤0.5）。在188个显著SNP附近，共鉴定出183个与一致性SNP和新SNP相关的候选基因。在这183个候选基因中，57个SNP存在于编码参与植物应激反应的生物学功能蛋白质的基因内。这些基因可能直接或间接与蒸腾作用或水分保持有关。已确认的基因组区域可能是聚合有利等位基因的重要资源，作为基因组选择的候选区域，可增强大豆的耐旱性。
