Natural and Life Sciences Faculty, Department of Biology, University of Oran, 1 Ahmed Ben Bella, Oran, Algeria.
Department of Epidemiology and Preventive Medicine, University Hospital of Oran (EHU), Oran, Algeria.
Parasitol Res. 2021 Sep;120(9):3195-3202. doi: 10.1007/s00436-021-07223-7. Epub 2021 Aug 3.
Human cystic echinococcosis is a zoonosis due to the flat worm Echinococcus granulosus sensu lato. The disease remains a major public health problem in Northern Africa. Molecular typing enables a better understanding of the parasite circulation from animals to humans. In this study, we investigated the genotypic diversity of 46 Echinococcus granulosus isolates collected from humans in the western part of Algeria by the mean of partial sequences of 4 mitochondrial loci, namely cox1a, cox1b, nd3, and atp6. Nucleotide polymorphism ranges from 0.6 (nd3) to 2.7% (cox1a). Eight alleles had not been previously reported. Multilocus analysis showed that all the isolates were from the Echinococcus granulosus sensu stricto (G1 genotype). Nineteen different haplotypes made of the concatenation of 4 sequenced loci were observed, the most common type clustering 13 isolates (36.1%). Twelve of these haplotypes had never been described previously and fifteen (41.7%) haplotypes were represented by only one isolate. Using sequences from this study and others retrieved from the GenBank database, any clustering either according to the geographic origin within Algeria or according to the human or animal origin of the isolates could be demonstrated supporting that genotype G1 population genetics has been shaped by intensive animal breeding.
人类包虫病是一种由细粒棘球绦虫（Echinococcus granulosus sensu lato）引起的人畜共患病。该病仍然是北非的一个主要公共卫生问题。分子分型有助于更好地了解寄生虫从动物到人类的循环。在这项研究中，我们通过 4 个线粒体基因座（cox1a、cox1b、nd3 和 atp6）的部分序列，调查了来自阿尔及利亚西部人类的 46 个细粒棘球蚴分离株的基因型多样性。核苷酸多态性范围从 0.6%（nd3）到 2.7%（cox1a）。有 8 种等位基因以前没有报道过。多基因座分析显示，所有分离株均来自细粒棘球蚴（Echinococcus granulosus sensu stricto）（G1 基因型）。观察到由 4 个测序基因座连接而成的 19 种不同的单倍型，最常见的类型聚类了 13 个分离株（36.1%）。其中 12 种单倍型以前从未描述过，15 种（41.7%）单倍型仅由一个分离株代表。使用本研究中的序列和从 GenBank 数据库中检索到的序列，可以根据阿尔及利亚境内的地理起源或根据分离株的人类或动物起源进行聚类，这表明 G1 基因型群体遗传学是由密集的动物养殖塑造的。
