Research Laboratory of Improvement and Development of Animal and Plant Production, Department of Biology and Animal Physiology, University Ferhat Abbas of Setif, Setif, Algeria.
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey.
Parasitol Res. 2024 Mar 11;123(3):159. doi: 10.1007/s00436-024-08176-3.
Cystic echinococcosis (CE) is a disease that can be transmitted from animals to humans, caused by the metacestode of Echinococcus granulosus. The disease has significant health and economic impacts worldwide, particularly in endemic areas. The study aimed to evaluate the prevalence of hydatid cysts in ruminants (cattle and sheep) (n = 2060) from the Setif Province of Algeria using microscopy. The results showed that hydatid cysts were detected in 9.6% (198/2060) of ruminants, with a higher prevalence in cattle (16.8%; 56/333) compared to sheep (8.2%; 142/1727). Molecular techniques were used to analyze a subset of animals consisting of 30 sheep and 4 cattle. Specifically, a fragment of the mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) gene was sequenced and compared to sequences from seven humans from the same region. The results indicated that all isolates were identified as E. granulosus sensu stricto. Haplotype analysis identified 19 E. granulosus s.s. haplotypes arranged like a star, with the dominant haplotype (Hap04) at the center. Hap04 has been assigned a total of 17 positives, including positives from sheep, cattle, and two humans. This study is noteworthy for being the first to use a molecular approach to human and ruminant echinococcosis in Setif, a significant breeding region in Algeria.
泡型包虫病（CE）是一种可以在动物和人类之间传播的疾病，由细粒棘球绦虫的囊尾蚴引起。该疾病在全球范围内对健康和经济都有重大影响，尤其是在流行地区。本研究旨在使用显微镜评估阿尔及利亚塞提夫省 2060 只反刍动物（牛和羊）（n=2060）中包虫囊肿的流行情况。结果显示，在 2060 只反刍动物中，有 9.6%（198/2060）检测到包虫囊肿，牛的患病率较高（16.8%；56/333），而绵羊的患病率较低（8.2%；142/1727）。使用分子技术对包括 30 只绵羊和 4 头牛在内的一小部分动物进行了分析。具体来说，对线粒体细胞色素 c 氧化酶亚单位 1（mt-CO1）基因的一个片段进行了测序，并与来自同一地区的 7 名人类的序列进行了比较。结果表明，所有分离株均被鉴定为细粒棘球绦虫亚种。单倍型分析鉴定出 19 种细粒棘球绦虫亚种单倍型，排列成星形，中心是优势单倍型（Hap04）。Hap04 共分配了 17 个阳性结果，包括来自绵羊、牛和两个人类的阳性结果。本研究是首次在阿尔及利亚重要的繁殖地区塞提夫使用分子方法对人和反刍动物的包虫病进行研究，具有重要意义。
