Samari Houssem, Laurimäe Teivi, Reghaissia Nassiba, Dahmane AbdEldjalil, Mamoune Ali, Baroudi Djamel, Deplazes Peter, Laatamna AbdElkarim
Higher National Veterinary School, Rue Issad Abbes, El Alia, Algiers 1600, Algeria; Laboratory of Exploration and Valorization of Steppic Ecosystems, Faculty of Nature and Life Sciences, University of Djelfa, Moudjbara Road, BP 3117, Djelfa, Algeria; Faculty of Sciences, University of Mohamed Boudiaf, BP 166, M'sila 2800, Algeria.
Institute of Parasitology, University of Zurich, Zürich, Switzerland; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Estonia.
Acta Trop. 2022 Oct;234:106616. doi: 10.1016/j.actatropica.2022.106616. Epub 2022 Jul 25.
Cystic echinococcosis is parasitic disease caused by the metacestodes belonging to the Echinococcus granulosus sensu lato (s.l.) species complex. Cystic echinococcosis is of considerable economic and public health importance. It is endemic in both livestock and humans in North African countries, including Algeria. The present study aimed to characterize E. granulosus s.l. genotypes in dromedary camels (Camelus dromedarius) from the extreme Sahara of Algeria, using recently developed mitochondrial genetic markers (NADH dehydrogenase subunit 2 and NADH dehydrogenase subunit 5) for reliable identification of different genotypes. A total of 75 Echinococcus cysts were collected from 49 dromedary camels, including 65 and 10 cysts from 45 and four camels originating from two slaughterhouses of Tindouf and Illizi provinces, respectively. E. granulosus sensu stricto (s.s.) G1 and G3 were identified in camels from both areas based on nad5 (649 bp) gene sequences, whereas E. granulosus s.l. G6 was identified in camels from Tindouf region based on concatenated nad5 and nad2 gene sequences (total 1336 bp). Identified samples clustered into 11 different haplotypes (ALG1-ALG11), including four haplotypes (ALG8-ALG11) for E. granulosus s.s. G1, one haplotype (ALG7) for E. granulosus s.s. G3, and six haplotypes (ALG1-ALG6) for E. granulosus s.l. G6. The present study provides valuable molecular data, including genotyping and haplotypic variability, on E. granulosus s.l. in dromedary camels from two regions in the extreme Sahara of Algeria. Future characterization of the G1, G3, and G6 samples based on sequencing of complete mitochondrial genomes would be of considerable significance for a more comprehensive understanding of molecular epidemiology of CE in Algeria.
囊型包虫病是由细粒棘球绦虫复合种的中绦期幼虫引起的寄生虫病。囊型包虫病具有相当大的经济和公共卫生重要性。在包括阿尔及利亚在内的北非国家，它在牲畜和人类中均为地方病。本研究旨在利用最近开发的线粒体遗传标记（烟酰胺腺嘌呤二核苷酸脱氢酶亚基2和烟酰胺腺嘌呤二核苷酸脱氢酶亚基5），对来自阿尔及利亚极端撒哈拉地区的单峰骆驼（骆驼属单峰驼）中的细粒棘球绦虫复合种基因型进行特征分析，以可靠地鉴定不同基因型。共从49峰单峰骆驼中收集到75个棘球绦虫囊肿，其中分别来自廷杜夫省和伊利齐省两个屠宰场的45峰和4峰骆驼的囊肿有65个和10个。基于烟酰胺腺嘌呤二核苷酸脱氢酶亚基5（649bp）基因序列，在两个地区的骆驼中均鉴定出了狭义细粒棘球绦虫G1和G3，而基于串联的烟酰胺腺嘌呤二核苷酸脱氢酶亚基5和烟酰胺腺嘌呤二核苷酸脱氢酶亚基2基因序列（共1336bp），在廷杜夫地区的骆驼中鉴定出了细粒棘球绦虫复合种G6。鉴定出的样本聚为11种不同的单倍型（ALG1 - ALG11），其中狭义细粒棘球绦虫G1有4种单倍型（ALG8 - ALG11），狭义细粒棘球绦虫G3有1种单倍型（ALG7），细粒棘球绦虫复合种G6有6种单倍型（ALG1 - ALG6）。本研究提供了关于阿尔及利亚极端撒哈拉地区两个区域单峰骆驼中细粒棘球绦虫复合种的有价值分子数据，包括基因分型和单倍型变异性。基于完整线粒体基因组测序对G1、G3和G6样本进行进一步特征分析，对于更全面地了解阿尔及利亚囊型包虫病的分子流行病学具有重要意义。
