School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
Department of Microbiology, Government College University, Faisalabad, Pakistan.
Crit Rev Eukaryot Gene Expr. 2021;31(2):55-62. doi: 10.1615/CritRevEukaryotGeneExpr.2021037677.
Sphingomonas sp. Shah is a bacterium that was first isolated from mammalian cell cultures. According to ribotyping data it is very much homologous to the clinically important pathogen Sphingomonas paucimobilis, which has generated pseudo-outbreaks. Using a tissue culture system, Sphingomonas sp. Shah was discovered to induce apoptosis in human lung epithelial carcinoma. Apoptosis of infected cells was determined by numerous criteria including (1) visual alterations in cellular morphology, (2) initiation of nuclear marginalization and chromatin compaction condensation, (3) the attendance of a high percentage of cells with subG1 DNA content, and (4) caspase-3 activation. In the current study we demonstrate the induction of apoptosis in mammalian lung epithelial cells upon infection with Sphingomonas sp. Shah and provide insight into the molecular processes triggering apoptosis.
沙氏鞘氨醇单胞菌是一种从哺乳动物细胞培养物中首次分离出来的细菌。根据核糖体分型数据，它与临床上重要的病原体少动鞘氨醇单胞菌非常同源，后者曾引发过假暴发。利用组织培养系统，发现沙氏鞘氨醇单胞菌可诱导人肺上皮癌细胞凋亡。感染细胞的凋亡通过多种标准确定，包括 (1) 细胞形态的视觉改变，(2) 核边缘化和染色质凝聚的启动，(3) 具有 subG1 DNA 含量的细胞比例高，以及 (4) caspase-3 激活。在本研究中，我们证明了沙氏鞘氨醇单胞菌感染诱导哺乳动物肺上皮细胞凋亡，并深入了解触发凋亡的分子过程。
