Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1576-1580. doi: 10.1099/ijs.0.031484-0. Epub 2011 Aug 26.
A novel bacterium (strain YIM 65583(T)) belonging to the genus Sphingomonas was isolated from surface-sterilized tissue of Artemisia annua L., which was collected from Yunnan province, south-west China. Its morphology, physiology and biochemical features were consistent with those of members of the genus Sphingomonas. Analysis of the 16S rRNA gene sequence of strain YIM 65583(T) further confirmed that it should be classified as a member of the genus Sphingomonas and was most closely related to Sphingomonas phyllosphaerae FA2(T) (99.7%) and Sphingomonas yunnanensis YIM 003(T) (98.3%). The isolate was Gram-negative and formed yellow-pigmented colonies on ISP 2 medium. It grew optimally at pH 6.0-8.0, at 20-37 °C and in the presence of 0-1% (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-10; C(18:1)ω7c, anteiso-C(16:1), C(14:0)-2OH, C(17:1)ω6c, C(16:0) and C(15:0) were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.3 mol%. The DNA-DNA relatedness values of the isolate YIM 65583(T) with S. phyllosphaerae FA2(T) and S. yunnanensis YIM 003(T) were 43.1% and 37.9%, respectively. Based on these features, it is concluded that the strain represents a novel species of the genus Sphingomonas, for which the name Sphingomonas endophytica sp. nov. is proposed, with YIM 65583(T) (=CCTCC AA 209035(T)=JCM 17394(T)) as the type strain.
一株新型细菌（菌株 YIM 65583(T)），属于鞘氨醇单胞菌属，从中国西南部云南省采集的青蒿表面消毒组织中分离得到。其形态、生理和生化特征与鞘氨醇单胞菌属的成员一致。菌株 YIM 65583(T)的 16S rRNA 基因序列分析进一步证实，它应归类为鞘氨醇单胞菌属，与鞘氨醇单胞菌属的 Phyllosphaerae FA2(T)（99.7%）和 Sphingomonas yunnanensis YIM 003(T)（98.3%）最为密切相关。该分离株为革兰氏阴性菌，在 ISP 2 培养基上形成黄色色素菌落。它在 pH6.0-8.0、20-37°C 和 0-1%（w/v）NaCl 存在下生长最佳。主要呼吸类脂醌是泛醌-10；C(18:1)ω7c、anteiso-C(16:1)、C(14:0)-2OH、C(17:1)ω6c、C(16:0)和 C(15:0)是主要的脂肪酸。主要极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和神经酰胺糖脂。基因组 DNA 的 G+C 含量为 63.3 mol%。菌株 YIM 65583(T)与 S. phyllosphaerae FA2(T)和 S. yunnanensis YIM 003(T)的 DNA-DNA 同源性值分别为 43.1%和 37.9%。基于这些特征，该菌株代表了鞘氨醇单胞菌属的一个新种，因此建议使用 Sphingomonas endophytica sp. nov. 这个名称，其模式菌株为 YIM 65583(T)（=CCTCC AA 209035(T)=JCM 17394(T)）。
