Wang Liang, Liu Wei, Tang Jia-Wei, Wang Jun-Jiao, Liu Qing-Hua, Wen Peng-Bo, Wang Meng-Meng, Pan Ya-Cheng, Gu Bing, Zhang Xiao
Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China.
Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, China.
Front Microbiol. 2021 Jul 19;12:683580. doi: 10.3389/fmicb.2021.683580. eCollection 2021.
Infectious diseases caused by bacterial pathogens are important public issues. In addition, due to the overuse of antibiotics, many multidrug-resistant bacterial pathogens have been widely encountered in clinical settings. Thus, the fast identification of bacteria pathogens and profiling of antibiotic resistance could greatly facilitate the precise treatment strategy of infectious diseases. So far, many conventional and molecular methods, both manual or automatized, have been developed for diagnostics, which have been proven to be accurate, reliable, and time efficient. Although Raman spectroscopy (RS) is an established technique in various fields such as geochemistry and material science, it is still considered as an emerging tool in research and diagnosis of infectious diseases. Based on current studies, it is too early to claim that RS may provide practical guidelines for microbiologists and clinicians because there is still a gap between basic research and clinical implementation. However, due to the promising prospects of label-free detection and noninvasive identification of bacterial infections and antibiotic resistance in several single steps, it is necessary to have an overview of the technique in terms of its strong points and shortcomings. Thus, in this review, we went through recent studies of RS in the field of infectious diseases, highlighting the application potentials of the technique and also current challenges that prevent its real-world applications.
由细菌病原体引起的传染病是重要的公共卫生问题。此外，由于抗生素的过度使用，临床上已广泛遇到许多耐多药细菌病原体。因此，快速鉴定细菌病原体并分析抗生素耐药性，可极大地促进传染病的精准治疗策略。到目前为止，已经开发了许多传统方法和分子方法用于诊断，这些方法既有手动的也有自动化的，已被证明准确、可靠且高效。尽管拉曼光谱（RS）在地球化学和材料科学等各个领域都是一项成熟的技术，但在传染病研究和诊断中仍被视为一种新兴工具。基于目前的研究，现在声称RS可为微生物学家和临床医生提供实用指南还为时过早，因为基础研究与临床应用之间仍存在差距。然而，由于RS在几个步骤中对细菌感染和抗生素耐药性进行无标记检测和非侵入性鉴定的前景广阔，有必要从其优点和缺点方面对该技术进行概述。因此，在本综述中，我们梳理了RS在传染病领域的最新研究，突出了该技术的应用潜力以及阻碍其实际应用的当前挑战。
