State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, China; Amoy Hopeful Biotechnology Co., Ltd., Xiamen, China.
Methods Enzymol. 2021;657:145-156. doi: 10.1016/bs.mie.2021.06.029. Epub 2021 Jul 13.
With the rapid development of biomedical imaging, non-invasive imaging method particularly has been widely used in clinical diagnosis. Different imaging methods have their own advantages, such as higher resolution of optical imaging, deeper penetration of acoustic imaging, high resolution photoacoustic imaging (PAI), and multi-parameter guidance of magnetic resonance imaging (MRI). Recent years, multimodal MRI, fluorescence imaging, PAI and others have been verified to play an important role in the field of molecular imaging and provided detail information for accurate in vivo diagnosis. Therefore, the design of multimodal probe that can integrate the above advantages to carry out combined imaging will be more accurate to assist diagnosis and treatment. While tumor microenvironment (TME) is highly critical in validating and optimizing current therapeutic strategies. Herein, we highlight the TME-triggered supramolecular system as an in situ nano-generator for multimodal imaging-guided treatment. The experimental protocols on the PAI/fluorescence imaging/MRI-based diagnosis are described in this chapter.
随着生物医学成像技术的快速发展，非侵入性成像方法尤其在临床诊断中得到了广泛应用。不同的成像方法各有优势，如光学成像是更高的分辨率，声学成像是更深的穿透性，光声成像是高分辨率，磁共振成像是多参数指导。近年来，多模态磁共振成像、荧光成像、光声成像等已被证实可在分子成像领域发挥重要作用，并为准确的体内诊断提供详细信息。因此，设计能够整合上述优势进行联合成像的多模态探针，将更有助于辅助诊断和治疗。而肿瘤微环境（TME）对于验证和优化当前的治疗策略至关重要。在此，我们重点介绍 TME 触发的超分子体系作为一种用于多模态成像引导治疗的原位纳米发生器。本章描述了基于光声成像/荧光成像/磁共振成像的诊断的实验方案。
