Bokkenheuser V D, Shackleton C H, Winter J
St. Luke's-Roosevelt Hospital Center, Pathology Department, New York, NY 10025.
Biochem J. 1987 Dec 15;248(3):953-6. doi: 10.1042/bj2480953.
Rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. The enzymes required for these hydrolyses, namely alpha-rhamnosidase and beta-galactosidase, were produced by some strains of Bacteroides distasonis; other strains, however, synthesized beta-glucosidase. The last-named enzyme was also elaborated by Bacteroides uniformis and Bacteroides ovatus. All the enzymes were produced constitutively. A cell-free extract of B. distasonis containing beta-glucosidase displayed an enzymic activity of 1 mumol/10 min per 10 mg of protein.
来自健康受试者的粪便菌群可将芦丁和槲皮苷水解为槲皮素，将刺槐素水解为山奈酚。这些水解所需的酶，即α-鼠李糖苷酶和β-半乳糖苷酶，由一些狄氏拟杆菌菌株产生；然而，其他菌株合成β-葡萄糖苷酶。后一种酶也由单形拟杆菌和卵形拟杆菌产生。所有这些酶都是组成型产生的。含有β-葡萄糖苷酶的狄氏拟杆菌无细胞提取物每10毫克蛋白质显示出1微摩尔/10分钟的酶活性。
