可逆磷酸化在介导大鼠妊娠和哺乳期肝脏3-羟基-3-甲基戊二酰辅酶A还原酶活性变化中的作用
Role of reversible phosphorylation in mediating changes in the activity of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase during pregnancy and lactation in the rat.
作者信息
Easom R A, Zammit V A
机构信息
Hannah Research Institute, Ayr, Scotland, U.K.
出版信息
Biochem J. 1987 Dec 15;248(3):993-6. doi: 10.1042/bj2480993.
Abstract
- The expressed and total (completely dephosphorylated) activities of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase were measured in microsomal fractions isolated from cold-clamped liver samples from female rats in various stages of the reproductive cycle. 2. There was little change in total HMG-CoA reductase activity during pregnancy and early lactation, but after 2 days post partum there was a marked increase in total activity. 3. The expressed/total activity ratio of HMG-CoA reductase showed a profound decrease during the last 2 days of pregnancy. The fraction of the enzyme in the active form increased progressively during the first 2 days of lactation. 4. The combined effect of these changes was that the expressed activity of HMG-CoA reductase changed in parallel with the known changes in the hepatic rate of cholesterogenesis during pregnancy and lactation in vivo.
摘要
- 测定了从处于生殖周期不同阶段的雌性大鼠冷钳夹肝脏样本中分离出的微粒体部分中3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶的表达活性和总（完全去磷酸化）活性。2. 妊娠和哺乳期早期，HMG-CoA还原酶的总活性变化不大，但产后2天后总活性显著增加。3. HMG-CoA还原酶的表达/总活性比值在妊娠的最后2天显著下降。在哺乳期的前2天，处于活性形式的酶比例逐渐增加。4. 这些变化的综合作用是，HMG-CoA还原酶的表达活性与体内妊娠和哺乳期肝脏胆固醇生成速率的已知变化平行变化。
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