Rothfield N, Whitaker D, Bordwell B, Weiner E, Senecal J L, Earnshaw W
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Arthritis Rheum. 1987 Dec;30(12):1416-9. doi: 10.1002/art.1780301214.
A solid-phase enzyme-linked immunosorbent assay has been established using a cloned fusion protein, CtermCENP-B [beta-gal], as antigen. The fusion protein carries the major epitope of CENP-B, the major centromeric autoantigen. The enzyme-linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud's disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.
已建立一种固相酶联免疫吸附测定法，使用克隆的融合蛋白CtermCENP-B [β-半乳糖苷酶]作为抗原。该融合蛋白携带CENP-B（主要着丝粒自身抗原）的主要表位。在检测硬皮病或雷诺病患者的抗着丝粒抗体方面，酶联免疫吸附测定法比免疫荧光技术更敏感，在3%的正常对照者和70例其他结缔组织病患者的3%中呈弱阳性。
