Rozek R J, Sherman M L, Liboff A R, McLeod B R, Smith S D
Industrial Health and Safety Program, Oakland University, Rochester, MI 48063.
Cell Calcium. 1987 Dec;8(6):413-27. doi: 10.1016/0143-4160(87)90025-x.
The incorporation of 45Ca in mixed human lymphocytes was measured following one-hour exposures of the cells to combined steady and periodic magnetic fields designed to probe for cyclotron resonance response in calcium incorporation. Measurements were made as a function of magnetic field frequency, up to 30 Hz, and as a function of magnetic field amplitude, up to 1.5 x 10(-4) Trms. The amplitude measurements demonstrated that the relative 45Ca uptake at resonance follows different mechanisms of interaction above and below 0.2 x 10(-4) Trms. After adjusting the magnetic field configuration for maximum incorporation, we then determined the effects of the calcium influx blocker nifedipine on 45Ca incorporation, with and without simultaneous exposure to this specific magnetic field combination. The presence of nifedipine in both unexposed and exposed cell suspensions resulted in decreased 45Ca uptake, presumably through the slow inward calcium channels. Evidence was found suggesting that nifedipine acts antagonistically to the 45Ca cyclotron resonance tuning signal.
在将人混合淋巴细胞暴露于旨在探测钙掺入中的回旋共振响应的稳态和周期性组合磁场一小时后，测量了45Ca在其中的掺入情况。测量是作为磁场频率（高达30Hz）和磁场幅度（高达1.5×10(-4)Trms）的函数进行的。幅度测量表明，在0.2×10(-4)Trms以上和以下，共振时相对45Ca摄取遵循不同的相互作用机制。在调整磁场配置以实现最大掺入后，我们接着确定了钙流入阻滞剂硝苯地平对45Ca掺入的影响，有无同时暴露于这种特定的磁场组合。在未暴露和暴露的细胞悬液中加入硝苯地平均导致45Ca摄取减少，推测是通过缓慢内向钙通道实现的。有证据表明硝苯地平对45Ca回旋共振调谐信号起拮抗作用。
