Montoya Daniel, D'Angelo Matilde, Martín-Orúe Susana M, Rodríguez-Sorrento Agustina, Saladrigas-García Mireia, Araujo Coralie, Chabrillat Thibaut, Kerros Sylvain, Castillejos Lorena
Animal Nutrition and Welfare Service (SNIBA), Animal and Food Science Department, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Phytosynthese, 57 Avenue Jean Jaurès, 63200 Mozac, France.
Animals (Basel). 2021 Jul 6;11(7):2024. doi: 10.3390/ani11072024.
This study evaluates the efficacy of two plant-based feed supplementations to fight colibacillosis in weanlings. A total of 96 piglets (32 pens) were assigned to four diets: a control diet (T1) or supplemented with ZnO (2500 ppm Zn) (T2) or two different plant supplements, T3 (1 kg/t; based on essential oils) and T4 (T3 + 1.5 kg/t based on non-volatile compounds). After one week, animals were challenged with ETEC F4, and 8 days after, one animal per pen was euthanized. Performance, clinical signs, microbial analysis, inflammatory response, intestinal morphology, and ileal gene expression were assessed. ZnO improved daily gains 4 days after challenge, T3 and T4 showing intermediate values (96, 249, 170, and 157 g/d for T1, T2, T3, and T4, = 0.035). Fecal lactobacilli were higher with T3 and T4 compared to ZnO (7.55, 6.26, 8.71, and 8.27 cfu/gFM; = 0.0007) and T3 increased the lactobacilli/coliforms ratio ( = 0.002). T4 was associated with lower levels of Pig-MAP ( = 0.07) and increases in villus/crypt ratio (1.49, 1.90, 1.73, and 1.84; = 0.009). Moreover, T4 was associated with an upregulation of the gene ( = 0.013; = 0.228) involved in the immune response induced by enteric pathogens. In conclusion, both plant supplements enhanced animal response in front of an ETEC F4 challenge probably based on different modes of action.
本研究评估了两种植物性饲料添加剂对断奶仔猪防治大肠杆菌病的效果。总共96头仔猪（32个栏）被分配到四种日粮中：对照日粮（T1）或添加氧化锌（2500 ppm锌）（T2）或两种不同的植物添加剂，T3（1 kg/t；基于精油）和T4（T3 + 1.5 kg/t基于非挥发性化合物）。一周后，用产肠毒素大肠杆菌F4对动物进行攻毒，攻毒8天后，每个栏处死一头动物。评估了生长性能、临床症状、微生物分析、炎症反应、肠道形态和回肠基因表达。氧化锌在攻毒4天后提高了日增重，T3和T4表现出中间值（T1、T2、T3和T4分别为96、249、170和157 g/d；P = 0.035）。与氧化锌相比，T3和T4组的粪便乳酸杆菌数量更高（7.55、6.26、8.71和8.27 cfu/gFM；P = 0.0007），且T3提高了乳酸杆菌/大肠杆菌比例（P = 0.002）。T4与较低水平的猪主要急性蛋白（P = 0.07）和绒毛/隐窝比值增加有关（1.49、1.90、1.73和1.84；P = 0.009）。此外，T4与参与肠道病原体诱导的免疫反应的基因上调有关（P = 0.013；P = 0.228）。总之，两种植物添加剂可能基于不同的作用方式增强了动物对产肠毒素大肠杆菌F4攻毒的反应。
