Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Departament of Microbiology and Biochemistry of Dairy Products, Paseo Río Linares s/n, 33300 Villaviciosa, Spain.
Division of Gastroenterology and Hepatology, and Microbiome Core, School of Medicine, Department of Medicine, University of North Carolina (UNC), Chapel Hill, NC 2759, USA.
Int J Environ Res Public Health. 2021 Jul 27;18(15):7939. doi: 10.3390/ijerph18157939.
Isoflavones are metabolized by components of the gut microbiota and can also modulate their composition and/or activity. This study aimed to analyze the modifications of the fecal microbial populations and their metabolites in menopausal women under dietary treatment with soy isoflavones for one month. Based on the level of urinary equol, the women had been stratified previously as equol-producers ( = 3) or as equol non-producers ( = 5). The composition of the fecal microbiota was assessed by high-throughput sequencing of 16S rRNA gene amplicons and the changes in fatty acid excretion in feces were analyzed by gas chromatography. A greater proportion of sequence reads of the genus was detected after isoflavone supplementation. Sequences of members of the family and the genus were significantly increased in samples from equol-producing women. Multivariable analysis showed that, after isoflavone treatment, the fecal microbial communities of equol producers were more like each other. Isoflavone supplementation increased the production of caproic acid, suggesting differential microbial activity, leading to a high fecal excretion of this compound. However, differences between equol producers and non-producers were not scored. These results may contribute to characterizing the modulating effect of isoflavones on the gut microbiota, which could lead to unravelling of their beneficial health effects.
异黄酮可被肠道微生物群的成分代谢，也可调节其组成和/或活性。本研究旨在分析食用大豆异黄酮一个月对绝经后妇女粪便微生物群及其代谢物的改变。根据尿中依普黄酮的水平，这些妇女先前被分为依普黄酮产生者（=3）或依普黄酮非产生者（=5）。通过 16S rRNA 基因扩增子高通量测序评估粪便微生物群的组成，并通过气相色谱分析粪便中脂肪酸的排泄变化。补充异黄酮后，属 的序列读长比例增加。在依普黄酮产生者的样本中，家族 和属 的成员序列显著增加。多变量分析表明，依普黄酮治疗后，依普黄酮产生者的粪便微生物群彼此更为相似。异黄酮补充增加了己酸的产生，提示微生物活性的差异，导致该化合物在粪便中的高排泄。然而，依普黄酮产生者和非产生者之间的差异并未被评分。这些结果可能有助于描述异黄酮对肠道微生物群的调节作用，这可能有助于揭示其有益的健康影响。
