Vázquez Lucía, Flórez Ana Belén, Guadamuro Lucía, Mayo Baltasar
Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Paseo Río Linares s/n, 33300 Villaviciosa, Spain.
Nutrients. 2017 Jul 8;9(7):727. doi: 10.3390/nu9070727.
The present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. To do so, the minimum inhibitory concentration (MIC) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. Additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (OD) of the cultures at 24 h were also determined. MICs of daidzin, genistin, daidzein, and genistein were >2048 μg mL for all strains assayed, while that of equol ranged from 16 μg mL for subsp. to >2048 μg mL for Enterobacteriaceae strains. Changes in growth curves, μ, and final OD were observed among the species in the presence of all tested compounds. Genistein reduced μ of , subsp. , and , while both genistein and equol increased that of and Compared to controls, lower final OD in the presence of aglycones and equol were recorded for some strains but were higher for others. Altogether, the results suggest that isoflavone-derived compounds could modify numbers of key bacterial species in the gut, which might be associated with their beneficial properties.
本研究旨在评估来自人类肠道的优势和代表性细菌群体对异黄酮及其代谢产物的敏感性。为此，通过肉汤微量稀释法测定了异黄酮糖苷、异黄酮苷元以及雌马酚对37种细菌菌株的最低抑菌浓度（MIC）。此外，对于10种代表性菌株，还测定了其生长曲线、生长速率（μ）以及培养24小时时的光密度（OD）。在所检测的所有菌株中，大豆苷、染料木苷、大豆苷元以及染料木素的MIC均>2048μg/mL，而雌马酚的MIC范围为：对于亚种为16μg/mL，对于肠杆菌科菌株则>2048μg/mL。在所有受试化合物存在的情况下，各物种的生长曲线、μ以及最终OD均出现了变化。染料木素降低了、亚种以及的μ，而染料木素和雌马酚均提高了和的μ。与对照相比，一些菌株在苷元及雌马酚存在时最终OD较低，但另一些菌株则较高。总体而言，结果表明异黄酮衍生化合物可能会改变肠道中关键细菌物种的数量，这可能与其有益特性相关。
