Wang Jia, Sheng Jiajing, Zhu Jianyong, Hu Zhongli, Diao Ying
School of Medicine, Anhui University of Science and Technology, Huainan, 232001 People's Republic of China.
College of Life Sciences, Nantong University, Nantong, 226019 People's Republic of China.
Physiol Mol Biol Plants. 2021 Jul;27(7):1499-1512. doi: 10.1007/s12298-021-01030-1. Epub 2021 Jul 2.
species are perennial C4 grasses that are considered promising energy crops because of their high biomass yields, excellent adaptability and low management costs. and are closely related subspecies that are distributed in different habitats. However, there are only a few reports on the mechanisms by which adapts to different environments. Here, comparative transcriptomic and morphological analyses were used to study the evolutionary adaptation of and to different habitats. In total, among 7586 identified orthologs, 2060 orthologs involved in phenylpropanoid biosynthesis and plant hormones were differentially expressed between the two species. Through an analysis of the Ka/Ks ratios of the orthologs, we estimated that the divergence time between the two species was approximately 4.37 Mya. In addition, 37 candidate positively selected orthologs (PSGs) that played important roles in the adaptation of these species to different habitats were identified. Then, the expression levels of 20 PSGs in response to flooding and drought stress were analyzed, and the analysis revealed significant changes in their expression levels. These results facilitate our understanding of the evolutionary adaptation to habitats and the speciation of and . We hypothesise that lignin synthesis genes are the main cause of the morphological differences between the two species. In summary, the plant nonspecific phospholipase C gene family and the receptor-like protein kinase gene family played important roles in the evolution of these two species.
The online version contains supplementary material available at 10.1007/s12298-021-01030-1.
该物种为多年生C4禾本科植物，因其生物量产量高、适应性强且管理成本低，被视为有前景的能源作物。[物种名称1]和[物种名称2]是密切相关的亚种，分布于不同栖息地。然而，关于[物种名称1]适应不同环境的机制的报道较少。在此，通过比较转录组学和形态学分析来研究[物种名称1]和[物种名称2]对不同栖息地的进化适应性。总共在7586个鉴定出的直系同源基因中，有2060个参与苯丙烷生物合成和植物激素的直系同源基因在这两个物种间差异表达。通过对直系同源基因的Ka/Ks比值分析，我们估计这两个物种的分化时间约为437万年前。此外，鉴定出37个在这些物种适应不同栖息地过程中起重要作用的候选正选择直系同源基因（PSGs）。然后，分析了20个PSGs在应对洪涝和干旱胁迫时的表达水平，分析结果显示它们的表达水平有显著变化。这些结果有助于我们理解对栖息地的进化适应性以及[物种名称1]和[物种名称2]的物种形成。我们假设木质素合成基因是这两个物种形态差异的主要原因。总之，植物非特异性磷脂酶C基因家族和类受体蛋白激酶基因家族在这两个物种的进化中发挥了重要作用。
在线版本包含可在10.1007/s12298-02:01030-1获取的补充材料。
