Lv Ting, Harris A J, Liu Yuping, Liu Tao, Liang Ruifang, Ma Zilan, Su Xu
School of Geosciences Qinghai Normal University Xining China.
Academy of Plateau Science and Sustainability Xining China.
Ecol Evol. 2021 Jul 13;11(15):10258-10276. doi: 10.1002/ece3.7831. eCollection 2021 Aug.
is an ecologically important desert grass that occurs in the Inner Mongolian Plateau where it is frequently the dominant species and is involved in sand stabilization and wind breaking. We sought to generate a preliminary demographic framework for to support the future studies of this species, its conservation, and sustainable utilization. To accomplish this, we characterized the genetic diversity and structure of 210 individuals from 43 natural populations of using amplified fragment length polymorphism (AFLP) markers. We obtained 1,728 well-defined amplified bands from eight pairs of primers, of which 1,654 bands (95.7%) were polymorphic. Results obtained from the AFLPs suggested effective alleles among populations of 1.32, a Nei's standard genetic distance value of 0.206, a Shannon index of 0.332, a coefficient of gene differentiation ( ) of 0.469, and a gene flow parameter (m) of 0.576. All these values indicate that there is abundant genetic diversity in , but limited gene flow. An analysis of molecular variance (AMOVA) showed that genetic variation mainly exists within populations (64.2%), and we found that the most genetically similar populations were often not geographically adjacent. Thus, this suggests that the mechanisms of gene flow are surprisingly complex in this species and may occur over long distances. In addition, we predicted the distribution dynamics of based on the spatial distribution modeling and found that its range has contracted continuously since the last interglacial period. We speculate that dry, cold climates have been critical in determining the geographic distribution of during the Quaternary period. Our study provides new insights into the population genetics and evolutionary history of in the Inner Mongolian Plateau and provides a resource that can be used to design in situ conservation actions and prioritize sustainable utilization.
是一种具有重要生态意义的荒漠草本植物，生长于内蒙古高原，常为优势物种，对固沙和防风有重要作用。我们试图为该物种构建一个初步的种群统计学框架，以支持未来对其进行的研究、保护及可持续利用。为此，我们利用扩增片段长度多态性（AFLP）标记对来自该物种43个自然种群的210个个体的遗传多样性和结构进行了表征。我们从八对引物中获得了1728条清晰的扩增条带，其中1654条（95.7%）具有多态性。AFLP分析结果表明，该物种种群间的有效等位基因数为1.32，Nei氏标准遗传距离值为0.206，香农指数为0.332，基因分化系数（）为0.469，基因流参数（m）为0.576。所有这些值表明该物种具有丰富的遗传多样性，但基因流有限。分子方差分析（AMOVA）表明，遗传变异主要存在于种群内部（64.2%），并且我们发现遗传上最相似的种群往往在地理上并不相邻。因此，这表明该物种的基因流机制出奇地复杂，可能发生在远距离之间。此外，我们基于空间分布模型预测了该物种的分布动态，发现自上一个间冰期以来其分布范围持续收缩。我们推测，干燥寒冷的气候在第四纪时期对该物种地理分布的确定起到了关键作用。我们的研究为内蒙古高原该物种的种群遗传学和进化历史提供了新的见解，并提供了一种可用于设计原地保护行动和确定可持续利用优先级的资源。
