Kasai Yoshiyuki, Morino Tsunetaro, Dobashi Izumi, Mori Eri, Yamamoto Kazuhisa, Kojima Hiromi
Department of Otorhinolaryngology, The Jikei University School of Medicine, Tokyo, Japan.
Front Bioeng Biotechnol. 2021 Jul 21;9:687946. doi: 10.3389/fbioe.2021.687946. eCollection 2021.
Temporary storage of nasal tissues and nasal cell sheets, which entails transportation between hospitals and cell culture facilities, is an important issue in regenerative medicine. Herein, we investigated the preservation of chilled and frozen nasal tissues and expiry dates of ready-to-use nasal cell sheets. Although the cell number in preserved tissues was lower than that in fresh tissue, nasal cell sheets could be fabricated from tissues that had been refrigerated for 5 days and frozen-thawed over 5 days. Moreover, the nasal mucosal cell sheets were preserved in a non-hazardous buffer. The cell number, viability, and structure were not maintained in saline containing E-cadherin for 2 days; however, these were maintained in Hank's balanced salt solution for 2 days, but not for 5 days. To assess the proliferation capacity of cells in the stored cell sheets, we performed cell sheet grafting assays . Cell sheets stored in Hank's balanced salt solution for 2 days adhered to collagen gel and expanded normally. Our results show that nasal tissues can be stored temporarily in refrigerators or deep freezers, and Hank's balanced salt solution can be used for preservation of ready-to-use cell sheets for a few days.
鼻组织和鼻细胞片的临时储存涉及医院与细胞培养设施之间的运输，这是再生医学中的一个重要问题。在此，我们研究了冷藏和冷冻鼻组织的保存以及即用型鼻细胞片的有效期。尽管保存组织中的细胞数量低于新鲜组织中的细胞数量，但鼻细胞片可以由冷藏5天和冻融5天以上的组织制成。此外，鼻黏膜细胞片保存在无害缓冲液中。含E-钙黏蛋白的盐水中细胞数量、活力和结构在2天内无法维持；然而，这些在汉克平衡盐溶液中可维持2天，但不能维持5天。为了评估储存细胞片中细胞的增殖能力，我们进行了细胞片移植试验。在汉克平衡盐溶液中储存2天的细胞片粘附在胶原凝胶上并正常扩展。我们的结果表明，鼻组织可以临时储存在冰箱或深冻箱中，汉克平衡盐溶液可用于将即用型细胞片保存几天。
