Medical Imaging Class 17-03, School of Medical Technology, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
Molecular Imaging Laboratory, School of Medical Technology, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
J Int Med Res. 2021 Aug;49(8):3000605211015079. doi: 10.1177/03000605211015079.
Primary liver cancer is one of the most common malignant tumors in China. Currently, immunotherapy for liver cancer is a research hotspot. Experimental studies and epidemiological investigations have confirmed the antineoplastic activity of low ionizing radiation. The aim of this study was to explore the optimal dose of low ionizing radiation to enhance immune function. Twenty-five New Zealand rabbits were randomly divided into five groups (n = 5 each): experimental group 1 (25 mGy), experimental group 2 (50 mGy), experimental group 3 (75 mGy), experimental group 4 (100 mGy), and the control group (0 mGy). VX-2 tumor tissue was injected into rabbits using a high-frequency B-ultrasound probe (3.5 MHz). Rabbits were irradiated, and on day 4 after irradiation, blood was collected from each rabbit. Blood chemistry, interleukin (IL)-4, interferon (IFN)-γ, immunoglobulin (Ig)G, and IgM levels were assessed. On day 15 after irradiation, macrophage phagocytic function was assessed. The rabbits were sacrificed, and the spleen was removed and weighed to calculate its spleen index. Each parameter was highest in the experimental group 3 (75 mGy). Thus, we suspect the optimal low ionizing radiation dose to improve immune function may be 75 mGy.
原发性肝癌是中国最常见的恶性肿瘤之一。目前，肝癌的免疫治疗是研究热点。实验研究和流行病学调查证实了低剂量电离辐射的抗肿瘤活性。本研究旨在探讨低剂量电离辐射增强免疫功能的最佳剂量。25 只新西兰兔随机分为 5 组（每组 5 只）：实验组 1（25 mGy）、实验组 2（50 mGy）、实验组 3（75 mGy）、实验组 4（100 mGy）和对照组（0 mGy）。使用高频 B 超探头（3.5 MHz）将 VX-2 肿瘤组织注入兔体内。对兔子进行照射，照射后第 4 天，从每只兔子中采集血液。评估血液化学、白细胞介素（IL）-4、干扰素（IFN）-γ、免疫球蛋白（Ig）G 和 IgM 水平。照射后第 15 天，评估巨噬细胞的吞噬功能。处死兔子，取出脾脏并称重，计算脾脏指数。实验组 3（75 mGy）的各项参数最高。因此，我们怀疑改善免疫功能的最佳低剂量电离辐射可能是 75 mGy。
