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SARS-CoV-2 感染过程中唾液 RT-PCR 敏感性的变化。

Change in Saliva RT-PCR Sensitivity Over the Course of SARS-CoV-2 Infection.

机构信息

Division of Infectious Diseases, Children's Hospital Los Angeles, Los Angeles, California.

Department of Pediatrics, University of Southern California, Los Angeles.

出版信息

JAMA. 2021 Sep 21;326(11):1065-1067. doi: 10.1001/jama.2021.13967.

DOI:10.1001/jama.2021.13967
PMID:34387653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8456387/
Abstract

This study investigates the timeframe that optimizes saliva sensitivity for SARS-CoV-2 detection using reverse transcriptase–polymerase chain reaction (RT-PCR) testing.

摘要

本研究旨在探讨使用逆转录-聚合酶链反应(RT-PCR)检测 SARS-CoV-2 时优化唾液灵敏度的时间范围。

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本文引用的文献

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2
Persistent SARS-CoV-2 RNA Shedding Without Evidence of Infectiousness: A Cohort Study of Individuals With COVID-19.持续的 SARS-CoV-2 RNA 脱落而无传染性证据:一项 COVID-19 患者的队列研究。
J Infect Dis. 2021 Oct 28;224(8):1362-1371. doi: 10.1093/infdis/jiab107.
3
Correlation Between 3790 Quantitative Polymerase Chain Reaction-Positives Samples and Positive Cell Cultures, Including 1941 Severe Acute Respiratory Syndrome Coronavirus 2 Isolates.3790份定量聚合酶链反应阳性样本与阳性细胞培养物之间的相关性,包括1941株严重急性呼吸综合征冠状病毒2分离株。
Clin Infect Dis. 2021 Jun 1;72(11):e921. doi: 10.1093/cid/ciaa1491.
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RT-qPCR assays based on saliva rather than on nasopharyngeal swabs are possible but should be interpreted with caution: results from a systematic review and meta-analysis.基于唾液而非鼻咽拭子的逆转录定量聚合酶链反应(RT-qPCR)检测是可行的,但应谨慎解读:一项系统评价和荟萃分析的结果
Acta Biomed. 2020 Sep 7;91(3):e2020025. doi: 10.23750/abm.v91i3.10020.
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Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages.唾液采样及其直接裂解,在供应短缺的情况下增加 SARS-CoV-2 诊断检测数量的绝佳选择。
J Clin Microbiol. 2020 Sep 22;58(10). doi: 10.1128/JCM.01659-20.