Department of Pharmaceutical Sciences, Faculty of Pharmacy, Philadelphia University, Amman 19392, Jordan.
Department of Basic Sciences, Faculty of Science, Philadelphia University, Amman 19392, Jordan.
Molecules. 2022 Feb 16;27(4):1334. doi: 10.3390/molecules27041334.
This study aimed at fabricating gold (Au), iron (Fe) and selenium (Se) nanoparticles (NPs) using various natural plant extracts from the Fertile Crescent area and evaluating their potential application as antioxidant and biocompatible agents to be used in the pharmaceutical field, especially in drug delivery. The Au-NPs were synthesized using and extracts, whereas the Fe-NPs and Se-NPs were synthesized using peel, fruit and seed extracts of . The phytofabricated NPs were characterized by the UV-visible spectroscopy, scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction (XRD) and energy-dispersive X-ray (EDS) spectroscopy. Scanning electron microscope technique showed that the synthesized NPs surface was spherical, and the particle size analysis confirmed a particle size of 50 nm. The crystalline nature of the NPs was confirmed by the XRD analysis. All synthesized NPs were found to be biocompatible in the fibroblast and human erythroleukemic cell lines. Se-NPs showed a dose-dependent antitumor activity as evidenced from the experimental results with breast cancer (MCF-7) cells. A dose-dependent, free-radical scavenging effect of the Au-NPs and Se-NPs was observed in the DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay, with the highest effect recorded for Au-NPs.
本研究旨在使用来自新月沃地地区的各种天然植物提取物来制备金（Au）、铁（Fe）和硒（Se）纳米粒子（NPs），并评估它们作为抗氧化剂和生物相容性试剂在制药领域，特别是在药物输送方面的潜在应用。Au-NPs 是使用 和 提取物合成的，而 Fe-NPs 和 Se-NPs 是使用 果皮、果实和种子提取物合成的。采用紫外-可见光谱、扫描电子显微镜、傅里叶变换红外光谱、X 射线衍射（XRD）和能谱（EDS）光谱对所合成的 NPs 进行了表征。扫描电子显微镜技术表明，所合成的 NPs 表面呈球形，粒径分析证实粒径为 50nm。XRD 分析证实了 NPs 的结晶性质。所有合成的 NPs 在成纤维细胞和人红白血病细胞系中均表现出良好的生物相容性。实验结果表明，Se-NPs 对乳腺癌（MCF-7）细胞具有剂量依赖性的抗肿瘤活性。在 DPPH（2,2-二苯基-1-苦基肼）测定中，观察到 Au-NPs 和 Se-NPs 的自由基清除作用呈剂量依赖性，其中 Au-NPs 的效果最高。
