Sasikala Manickavasagam, Mohan Sellappan, Karuppaiah Arjunan, Karthick Vedi, Ragul Palanigoundar Atheyannan, Nagarajan Arumugam
Karpagam College of Pharmacy, Coimbatore 641 032 Tamil Nadu, India; The Tamil Nadu Dr. M.G.R. Medical University, Chennai 600032 Tamil Nadu, India.
Karpagam College of Pharmacy, Coimbatore 641 032 Tamil Nadu, India; The Tamil Nadu Dr. M.G.R. Medical University, Chennai 600032 Tamil Nadu, India; PSG College of Pharmacy, Peelamedu, Avinashi Road, Coimbatore 641004, India.
J Genet Eng Biotechnol. 2025 Mar;23(1):100470. doi: 10.1016/j.jgeb.2025.100470. Epub 2025 Feb 28.
Improving the pharmacokinetics of drugs is achieved through nano formulations and the role of natural product in the synthesis of nanomaterials is gaining prominence due to its eco-friendly nature, cost-effectiveness, and demonstrated efficacy. Metal nanoparticles (NPs) derived from Ipomoea aquatica Forsskal have been synthesized and evaluated for their antioxidant and antidiabetic properties towards enhancing the anticancer activity of the plant extracts.
Hydroalcoholic extract was obtained from the entire Ipomoea aquatica plant and utilized as a key ingredient in the green synthesis of metal NPs. The characterization of the synthesized NPs involved UV/visible and FT-IR spectroscopic analyses, along with particle size determination using Zetasizer technology. Antioxidant activity was assessed through DPPH radical scavenging assays, while antidiabetic potential was evaluated via alpha-amylase inhibitory activity using HPTLC bioautography.
The formation of silver nanoparticles (AgNPs) was confirmed by a color change from light brown to dark brown. UV-VIS spectrum analysis showed strong absorbance between 380 and 400 nm, with a peak at 428 nm, indicating successful synthesis via bioreduction by Ipomoea aquatica extract. FT-IR spectra revealed phytochemicals like flavonoids and proteins, with shifts in peak positions confirming AgNP formation. DLS showed an average particle size of 36.27 nm, and TEM images confirmed spherical morphology. The AgNPs exhibited significant antioxidant and antidiabetic activities, outperforming standards such as ascorbic acid and Glibenclamide. Toxicity prediction identified the extract as slightly toxic, guiding safe dose administration.
The study underscores the potential of plant-based nanoparticles in scavenging free radicals and supporting cytotoxicity, thus hinting at their potential role in cancer therapy. Moreover, the nanoparticles derived from Ipomoea aquatica exhibit promising antioxidant and antidiabetic activities compared to the crude plant extract. This research paves the way for further exploration of Ipomoea aquatica nanoparticles as a novel therapeutic intervention for various diseases.
通过纳米制剂可改善药物的药代动力学，并且由于其环保特性、成本效益和已证实的功效，天然产物在纳米材料合成中的作用正日益突出。已合成了源自蕹菜的金属纳米颗粒（NPs），并评估了它们对增强植物提取物抗癌活性的抗氧化和抗糖尿病特性。
从整个蕹菜植株中获得水醇提取物，并将其用作金属纳米颗粒绿色合成中的关键成分。合成的纳米颗粒的表征涉及紫外/可见和傅里叶变换红外光谱分析，以及使用Zetasizer技术测定粒径。通过DPPH自由基清除试验评估抗氧化活性，同时使用HPTLC生物自显影通过α-淀粉酶抑制活性评估抗糖尿病潜力。
银纳米颗粒（AgNPs）的形成通过颜色从浅棕色变为深棕色得以证实。紫外-可见光谱分析显示在380至400nm之间有强吸收，在428nm处有一个峰值，表明通过蕹菜提取物的生物还原成功合成。傅里叶变换红外光谱显示了黄酮类化合物和蛋白质等植物化学物质，峰位置的变化证实了AgNP的形成。动态光散射显示平均粒径为36.27nm，透射电子显微镜图像证实为球形形态。AgNPs表现出显著的抗氧化和抗糖尿病活性，优于抗坏血酸和格列本脲等标准品。毒性预测确定该提取物有轻微毒性，为安全给药剂量提供了指导。
该研究强调了植物基纳米颗粒在清除自由基和支持细胞毒性方面的潜力，从而暗示了它们在癌症治疗中的潜在作用。此外，与粗植物提取物相比，源自蕹菜的纳米颗粒表现出有前景的抗氧化和抗糖尿病活性。这项研究为进一步探索蕹菜纳米颗粒作为各种疾病的新型治疗干预措施铺平了道路。
