Xu Tong, Liu Lele, Shi Chenjuan, Liu Wenkai, Wang Min, Tian Li, Zheng Ye, Wang Hongmei, Zheng Wenwen, He Hongbin, Xia Xianzhu, Zheng Xuexing
Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.
Department of Biological Sciences, College of Life Sciences, Shandong Normal University, Jinan, China.
Transbound Emerg Dis. 2022 Jul;69(4):e254-e266. doi: 10.1111/tbed.14292. Epub 2021 Aug 26.
Cystic echinococcosis (CE), caused by Echinococcus granulosus (E, is a zoonosis with a worldwide distribution, resulting in heavy impact to public health and social economics. In this study, we generated a recombinant rabies virus (RABV) expressing EG95 protein of E. granulosus (LBNSE-EG95) as a bivalent candidate vaccine for use in sheep and cattle against CE and rabies, which is another severe health threat in CE-endemic areas. It was found that EG95 was successfully expressed without altering the pathogenicity of parent LBNSE vector. Further study showed that LBNSE-EG95 immunization in mice elicited activation of dendric cells (DCs) and B cells and induced Th1-/Th2-mediated cellular immune responses, leading to robust production of RABV neutralizing antibodies and high level of EG95-sepecific antibodies with more than 90% protection against CE. In addition, single dose of LBNSE-EG95 conferred full protection against lethal RABV challenge in mice. Collectively, these results suggest that the recombinant LBNSE-EG95 has the potential to be developed as an efficient bivalent vaccine for sheep and cattle use in endemic areas of CE and rabies.
囊型包虫病（CE）由细粒棘球绦虫（E）引起，是一种分布于全球的人畜共患病，对公众健康和社会经济造成严重影响。在本研究中，我们构建了一种表达细粒棘球绦虫EG95蛋白的重组狂犬病病毒（RABV）（LBNSE-EG95），作为一种双价候选疫苗用于绵羊和牛，以预防CE和狂犬病，狂犬病是CE流行地区的另一种严重健康威胁。结果发现EG95成功表达，且未改变亲本LBNSE载体的致病性。进一步研究表明，LBNSE-EG95免疫小鼠可激活树突状细胞（DCs）和B细胞，并诱导Th1/Th2介导的细胞免疫反应，从而产生大量的RABV中和抗体和高水平的EG95特异性抗体，对CE的保护率超过90%。此外，单剂量的LBNSE-EG95可使小鼠完全抵抗致死性RABV攻击。总体而言，这些结果表明重组LBNSE-EG95有潜力被开发为一种高效的双价疫苗，用于CE和狂犬病流行地区的绵羊和牛。
