Achadu Ojodomo J, Abe Fuyuki, Hossain Farzana, Nasrin Fahmida, Yamazaki Masahito, Suzuki Tetsuro, Park Enoch Y
Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan.
Department of Microbiology, Shizuoka Institute of Environment and Hygiene, 232-1, Yainaba, Fujieda, 426-0083, Japan.
Biosens Bioelectron. 2021 Dec 1;193:113540. doi: 10.1016/j.bios.2021.113540. Epub 2021 Aug 3.
Synergistic dual-mode optical platforms are up-and-coming detection tools in the diagnosis and management of infectious diseases. Here, novel dual-modality fluorescence (FL) and surface-enhanced Raman scattering (SERS) techniques have been integrated into a single probe for the rapid and ultrasensitive detection of norovirus (NoV). The developed FL-SER-based biosensor relies on the dual-signal enhancements of newly synthesized sulfur-doped agar-derived carbon dots (S-agCDs). The antigen-antibody immunoreaction results in forming a core-satellite immunocomplex between anti-NoV antibody-conjugated S-agCDs and polydopamine-functionalized magnetic silver nanocubes [poly (dop)-MNPs-Ag NCs]. By deploying an immunomagnetic enrichment protocol and performing the SERS modality on a single-layer graphene substrate, norovirus-like particles (NoV-LPs) were detected across a wide range of 1 fg mL - 10 ng mL with an excellent limit of detection of 0.1 fg mL. The combined advantage of the dual-signaling properties of the biosensor was demonstrated using FL confocal imaging for "hotspots" tracking prior to SERS detection of clinical NoV in fecal specimen down to ⁓10 RNA copies mL. The proposed dual-modality biosensor's performance increases the prospect of a rapid and low-cost sensitive NoV detection and surveillance option for public health.
协同双模式光学平台是传染病诊断和管理中新兴的检测工具。在此，新型双模态荧光（FL）和表面增强拉曼散射（SERS）技术已集成到单个探针中，用于快速、超灵敏地检测诺如病毒（NoV）。所开发的基于FL-SER的生物传感器依赖于新合成的硫掺杂琼脂衍生碳点（S-agCDs）的双信号增强。抗原-抗体免疫反应导致抗NoV抗体偶联的S-agCDs与聚多巴胺功能化磁性银纳米立方体[聚（多巴胺）-MNPs-Ag NCs]之间形成核-卫星免疫复合物。通过采用免疫磁富集方案并在单层石墨烯基板上进行SERS检测，在1 fg mL - 10 ng mL的宽范围内检测到诺如病毒样颗粒（NoV-LPs），检测限低至0.1 fg mL。在对粪便标本中的临床NoV进行SERS检测之前，使用FL共聚焦成像对“热点”进行追踪，证明了生物传感器双信号特性的综合优势，检测下限低至约10个RNA拷贝mL。所提出的双模态生物传感器的性能增加了为公共卫生提供快速、低成本灵敏NoV检测和监测选项的前景。
