Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Endocrinology and Metabolism, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Front Cell Infect Microbiol. 2021 Jan 22;10:575084. doi: 10.3389/fcimb.2020.575084. eCollection 2020.
Type 2 diabetes is a complex metabolic disease and has been shown to involve alteration of the gut microbiota. Previous studies have primarily focused on changes in the bacterial microbiome, while ignoring the phage community composition. Extracellular phages can lyse host bacteria and thus influence the microbiota through positive or negative interactions with bacteria. We investigated changes in the extracellular phageome and discussed its role in T2D pathogenesis. We used a sequencing-based approach to identify bacteriophage after isolation of VLPs (virus like particles) from fecal samples. We identified 330 species of phages according to the predicted host bacteria from T2D patients (N=17) and nondiabetic controls (N=29). The phageome characteristics were highly diverse among individuals. In the T2D group, the intestinal phage population was altered, and the abundance of phages specific to hosts increased markedly. Meanwhile, the abundance of in the gut was significantly increased, and systemic LPS content elevation was observed in the T2D group. Additionally, a consortia of eight phages was found to distinguish T2D patients from nondiabetic controls with good performance (AUC>0.99).
2 型糖尿病是一种复杂的代谢性疾病，已被证明与肠道微生物群的改变有关。以前的研究主要集中在细菌微生物组的变化上，而忽略了噬菌体群落组成。细胞外噬菌体可以裂解宿主细菌，因此通过与细菌的正相互作用或负相互作用来影响微生物群。我们研究了细胞外噬菌体组的变化，并讨论了其在 T2D 发病机制中的作用。我们使用基于测序的方法来鉴定从粪便样本中分离的 VLPs（病毒样颗粒）中的噬菌体。我们根据来自 T2D 患者（N=17）和非糖尿病对照（N=29）的预测宿主细菌鉴定了 330 种噬菌体。噬菌体组特征在个体之间高度多样化。在 T2D 组中，肠道噬菌体群发生改变，并且特定于宿主的噬菌体丰度显著增加。同时，在肠道中观察到 丰度显著增加，并且 T2D 组中存在全身 LPS 含量升高。此外，发现一组由 8 种噬菌体组成的噬菌体可以很好地区分 T2D 患者和非糖尿病对照（AUC>0.99）。
