分析波兰前列腺癌患者的氧化应激指标。

Analysis of oxidative stress indicators in Polish patients with prostate cancer.

机构信息

Department of Biotechnology, Kazimierz Wielki University, Księcia Józefa Poniatowskiego St.12, PL 85-671, Bydgoszcz, Poland.

Collegium Medicum in Bydgoszczy, Faculty of Medicine, Department of Medical Biology and Biochemistry, Department of Ecology and Environmental Protection, Nicolaus Copernicus University in Toruń, M. Curie Skłodowskiej St.9, PL 85-094, Bydgoszcz, Poland.

出版信息

Environ Sci Pollut Res Int. 2022 Jan;29(3):4632-4640. doi: 10.1007/s11356-021-15922-y. Epub 2021 Aug 19.

DOI:10.1007/s11356-021-15922-y

PMID:34409535

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8741701/

Abstract

The aim of the study was to analyze the activity of antioxidant enzymes (glutathione S-transferase, catalase, superoxide dismutase) and the concentration of malondialdehyde in order to determine the role of detoxification mechanisms in prostate cancer. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method. Superoxide dismutase was the only enzyme among antioxidant and detoxification enzymes for which a statistically significant difference in activity was found between the studied groups (1.4 U·ml in patients vs. 1.6 U·ml in control). No statistically significant differences were found for GST, CAT or the concentration of MDA between the group of men with prostate cancer and the control group. The lower SOD activity in men with prostate cancer may be due to a deficiency in their antioxidant defense system.

摘要

本研究旨在分析抗氧化酶（谷胱甘肽 S-转移酶、过氧化氢酶、超氧化物歧化酶）的活性和丙二醛的浓度，以确定解毒机制在前列腺癌中的作用。使用现成的试剂盒测量超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽 S-转移酶（GST）的活性；通过硫代巴比妥酸法测定脂质过氧化强度。在抗氧化和解毒酶中，只有超氧化物歧化酶的活性在研究组之间存在统计学差异（患者组为 1.4 U·ml，对照组为 1.6 U·ml）。在前列腺癌组和对照组之间，GST、CAT 或 MDA 浓度均无统计学差异。前列腺癌患者 SOD 活性较低可能是由于其抗氧化防御系统不足。