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从环境污水中分离和鉴定O157:H7裂解性噬菌体

Isolation and Identification of O157:H7 Lytic Bacteriophage from Environment Sewage.

作者信息

Sjahriani Tessa, Wasito Eddy Bagus, Tyasningsih Wiwiek

机构信息

Doctoral Program, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road no. 47, Surabaya 60285, Indonesia.

Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road no. 47, Surabaya 60285, Indonesia.

出版信息

Int J Food Sci. 2021 Aug 11;2021:7383121. doi: 10.1155/2021/7383121. eCollection 2021.

DOI:10.1155/2021/7383121
PMID:34423027
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8376447/
Abstract

O157:H7 is one of the pathogenic bacteria causing foodborne disease. The use of lytic bacteriophages can be a good solution to overcome the disease. This study is aimed at isolating lytic bacteriophages from environmental sewage with O157:H7 bacterial cells. The sample used in this study was eight bacteriophages, and the technique used in identifying O157:H7 carriers of the 1 and 2 genes was PCR. The double layer plaque technique was used to classify bacteriophages. Plaque morphology, host specificity, and electron micrograph were used to identify the bacteriophages. The result obtained plaque morphology as a clear zone with the largest diameter size of 3.5 mm. Lytic bacteriophage could infect O157:H7 at the highest titer of 10 × 10 PFU/mL. Bacteriophages have been identified as and . Phage 3, phage 4, and phage 8 could infect 1 (1) due to their host specificity. The Friedman statistical tests indicate that lytic bacteriophage can significantly lyse O157:H7 ( = 0.012). The lysis of O157:H7 by phage 1, phage 2, phage 3, and phage 5 bacteriophages was statistically significant, according to Conover's posthoc test ( < 0.05). The conclusion obtained from this study is that lytic bacteriophages from environmental sewage could lyse O157:H7. Therefore, it could be an alternative biocontrol agent against O157:H7 that contaminates food causing foodborne disease.

摘要

O157:H7是引起食源性疾病的病原菌之一。使用裂解性噬菌体可能是克服该疾病的一个好办法。本研究旨在从含有O157:H7细菌细胞的环境污水中分离裂解性噬菌体。本研究使用的样本是八种噬菌体，用于鉴定1和2基因的O157:H7携带者的技术是聚合酶链反应（PCR）。双层平板技术用于对噬菌体进行分类。噬菌斑形态、宿主特异性和电子显微镜照片用于鉴定噬菌体。得到的结果是噬菌斑形态为一个清晰区域，最大直径尺寸为3.5毫米。裂解性噬菌体能够以最高效价10×10 PFU/mL感染O157:H7。噬菌体已被鉴定为 和 。由于宿主特异性，噬菌体3、噬菌体4和噬菌体8能够感染1(1)。弗里德曼统计检验表明裂解性噬菌体能够显著裂解O157:H7（P = 0.012）。根据康诺弗事后检验（P < 0.05），噬菌体1、噬菌体2、噬菌体3和噬菌体5对O157:H7的裂解具有统计学意义。本研究得出的结论是，来自环境污水的裂解性噬菌体能够裂解O157:H7。因此，它可能是一种针对污染食物导致食源性疾病的O157:H7的替代生物防治剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/693e2564266c/IJFS2021-7383121.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/f4347bc3fd62/IJFS2021-7383121.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/4b399046343c/IJFS2021-7383121.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/b101761e0613/IJFS2021-7383121.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/e456106da191/IJFS2021-7383121.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/78a2e3653391/IJFS2021-7383121.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/693e2564266c/IJFS2021-7383121.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/f4347bc3fd62/IJFS2021-7383121.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/4b399046343c/IJFS2021-7383121.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/b101761e0613/IJFS2021-7383121.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/e456106da191/IJFS2021-7383121.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/78a2e3653391/IJFS2021-7383121.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dfa/8376447/693e2564266c/IJFS2021-7383121.006.jpg

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