Department of Chemistry, Institute for Applied Chemistry, Hallym University, Chuncheon 200-702, Korea.
Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea.
Biosensors (Basel). 2021 Aug 6;11(8):265. doi: 10.3390/bios11080265.
Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 10 to 10 copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT.
高灵敏度（高 SBR）和高特异性（高 SNP 区分率）的 DNA 杂交对于具有临床应用的生物传感器至关重要。在此，我们提出了一种方法，该方法可以在 10 至 10 拷贝/测试的动态范围内实现超过 160:1 的 SNP 区分率，从而在单个平台上检测多种病原体。新开发的 SWAT 方法可以实现高灵敏度和高特异性的 DNA 杂交。SWAT 在临床样本中对 MTB 和 NTM 菌株的检测和区分，其 SBR 和 SNP 区分率均高于 160:1，表明其具有很高的临床适用性。
