Department of Stem Cell and Regenerative Biotechnology, Konkuk Institute of Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.
Cells. 2021 Jul 26;10(8):1889. doi: 10.3390/cells10081889.
Assisted reproductive technologies (ARTs) have developed considerably in recent years; however, they cannot rectify germ cell aplasia, such as non-obstructive azoospermia (NOA) and oocyte maturation failure syndrome. In vitro gametogenesis is a promising technology to overcome infertility, particularly germ cell aplasia. Early germ cells, such as primordial germ cells, can be relatively easily derived from pluripotent stem cells (PSCs); however, further progression to post-meiotic germ cells usually requires a gonadal niche and signals from gonadal somatic cells. Here, we review the recent advances in in vitro male and female germ cell derivation from PSCs and discuss how this technique is used to understand the biological mechanism of gamete development and gain insight into its application in infertility.
近年来，辅助生殖技术（ART）有了相当大的发展；然而，它们无法纠正生殖细胞发育不全，如非梗阻性无精子症（NOA）和卵母细胞成熟失败综合征。体外配子发生是克服不孕的一项有前途的技术，特别是生殖细胞发育不全。原始生殖细胞等早期生殖细胞可以相对容易地从多能干细胞（PSCs）中获得；然而，进一步进展到减数分裂后生殖细胞通常需要生殖嵴和来自生殖嵴体细胞的信号。在这里，我们回顾了从 PSCs 体外获得雄性和雌性生殖细胞的最新进展，并讨论了该技术如何用于理解配子发育的生物学机制，并深入了解其在不孕中的应用。
