Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
Department of Entomology, National Taiwan University, Taipei 10617, Taiwan.
Viruses. 2021 Jul 23;13(8):1434. doi: 10.3390/v13081434.
Tomato () is an important economic crop worldwide. However, tomato production is jeopardized by the devastating tomato yellow leaf curl disease caused by whitefly-transmitted begomoviruses (WTBs). In this study, we evaluated the efficacy of our previously developed plant antiviral immunity inducer, fungal F8-culture filtrate, on tomato to combat tomato yellow leaf curl Thailand virus (TYLCTHV), the predominant WTB in Taiwan. Our results indicated that F8-culture filtrate treatment induced strong resistance, did not reduce the growth of tomato, and induced prominent resistance against TYLCTHV both in the greenhouse and in the field. Among TYLCTHV-inoculated Yu-Nu tomato grown in the greenhouse, a greater percentage of plants treated with F8-culture filtrate (43-100%) were healthy-looking compared to the HO control (0-14%). We found that TYLCTHV cannot move systemically only on the F8-culture filtrate pretreated healthy-looking plants. Tracking the expression of phytohormone-mediated immune maker genes revealed that F8-culture filtrate mainly induced salicylic acid-mediated plant immunity. Furthermore, callose depositions and the expression of the pathogen-induced callose synthase gene, were only strongly induced by TYLCTHV on tomato pretreated with F8-culture filtrate. This study provides an effective way to induce tomato resistance against TYLCTHV.
番茄（）是世界范围内重要的经济作物。然而，由粉虱传播的番茄黄曲叶病毒（WTBs）引起的毁灭性番茄黄曲叶卷曲病威胁着番茄的生产。在本研究中，我们评估了我们之前开发的植物抗病毒免疫诱导剂，真菌 F8 培养滤液，对番茄防治台湾主要 WTB 番茄黄曲叶卷曲泰国病毒（TYLCTHV）的功效。我们的结果表明，F8 培养滤液处理诱导了强烈的抗性，不会降低番茄的生长，并且在温室和田间都诱导了对 TYLCTHV 的明显抗性。在温室中种植的 Yu-Nu 番茄中，用 F8 培养滤液处理的植物（43-100%）比 HO 对照（0-14%）中有更多看起来健康的植物。我们发现 TYLCTHV 不能仅在预处理过的看起来健康的 F8 培养滤液植物上系统性移动。跟踪植物激素介导的免疫标志物基因的表达表明，F8 培养滤液主要诱导水杨酸介导的植物免疫。此外，仅在用 F8 培养滤液预处理的番茄上，才会强烈诱导胼胝质沉积和病原体诱导的胼胝质合酶基因的表达。本研究为诱导番茄对 TYLCTHV 的抗性提供了一种有效方法。
