Marine College, Shandong University, Weihai, 264209, China.
Arch Microbiol. 2021 Nov;203(9):5613-5619. doi: 10.1007/s00203-021-02543-9. Epub 2021 Aug 28.
A Gram-stain negative, non-flagellated, beige-pigmented, circular, catalase-positive, oxidase-positive bacterium, designated G4, was isolated from gut microflora of top shell (Trochus maculatus Linnaeus) collected from Diwanggong market, Weihai, People's Republic of China. The novel isolate was able to grow at 4-42 °C (optimum 25-33 °C), pH 7.0-9.0 (optimum 6.5-7.0) and with 0.0-11.0% NaCl (optimum 2.0-3.0%, w/v). Analysis of 16S rRNA gene sequence revealed that strain G4 shared the highest 16S rRNA gene sequence similarities with Oceaniglobus ichthyenteri YLY08 (96.6%), followed by Oceaniglobus indicus 1-19b (95.3%). The genome of strain G4, with 32 assembled contigs, was 4.5 Mb long with a G+C content of 65.3 mol%. DNA-DNA hybridization values of the isolate against the closely related type strains were far below the 70% limit for species delineation. The average amino acid identity, average nucleotide identity and digital DNA-DNA genome hybridization relatedness between strain G4 and the closely related members of the genus Oceaniglobus, Oceaniglobus indicus1-19b and Oceaniglobus ichthyenteri YLY08 were 71.3, 76.4 and 20.0%, and 75.0, 76.3 and 19.4%. The major cellular fatty acid was summed feature 8 (Cω7c and/or Cω6c). The sole respiratory quinone was Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyldimethylethanolamine. The results of phenotypical, phylogenetic and biochemical analyses indicated that strain G4 represents a novel species in genus Oceaniglobus within the family Rhodobacteraceae, for which the name Oceaniglobus trochenteri sp. nov. is proposed. The type strain is G4 (= MCCC 1K04356 = KCTC 82506).
一株革兰氏阴性、无鞭毛、米色、圆形、触酶阳性、氧化酶阳性的细菌，被命名为 G4，从中国威海帝王宫市场收集的巨凤螺肠道微生物群中分离得到。该新分离株能够在 4-42°C（最适温度 25-33°C）、pH 值 7.0-9.0（最适 pH 值 6.5-7.0）和 0.0-11.0%NaCl（最适 2.0-3.0%，w/v）的条件下生长。16S rRNA 基因序列分析表明，菌株 G4 与海洋小球藻 YLY08 的 16S rRNA 基因序列相似性最高（96.6%），其次是印度海洋球菌 1-19b（95.3%）。菌株 G4 的基因组由 32 个组装的连续序列组成，长 4.5 Mb，GC 含量为 65.3 mol%。该分离株与密切相关的模式菌株的 DNA-DNA 杂交值远低于种界定的 70%限值。菌株 G4 与密切相关的海洋球菌属成员海洋小球藻 YLY08、印度海洋球菌 1-19b 的平均氨基酸同一性、平均核苷酸同一性和数字 DNA-DNA 基因组杂交相关性分别为 71.3%、76.4%和 20.0%，以及 75.0%、76.3%和 19.4%。主要细胞脂肪酸为 sumFeature 8（Cω7c 和/或 Cω6c）。唯一的呼吸醌为 Q-10。极性脂谱由双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰二甲乙醇胺组成。表型、系统发育和生化分析的结果表明，菌株 G4 代表红杆菌科海洋球菌属中的一个新种，建议将其命名为海洋小球藻 trochenteri sp. nov。模式菌株为 G4（=MCCC 1K04356=KCTC 82506）。
