Wakene Wakuma, Asmamaw Solomon, Kahaliw Wubayehu
Department of Pharmacy, College of Health Sciences, Mettu University, Mettu, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
J Exp Pharmacol. 2021 Aug 22;13:859-872. doi: 10.2147/JEP.S318696. eCollection 2021.
Currently, there is a demand for new antidiabetic drugs from natural therapeutic agents, and diabetes mellitus disease is global epidemic. The leaf of has been used in the traditional health system for the management of diabetes mellitus in Ethiopia and Eritrea. The aim of this study was to evaluate in vivo antidiabetic, antihyperlipidemic and in vitro antioxidant activity of leaf extract and solvent fractions of the leaf of (Vahl) in mice.
The blood glucose-lowering activities of leaf extract and solvent fractions of the leaf of were screened in the normoglycemic, glucose loaded, and streptozotocin-induced diabetic mice models. In the treatment of streptozotocin-induced diabetic mice with leaf extract and solvent fractions, weight and lipid profile was measured. Antioxidant activity of the plant leaf extract was determined using DPPH assay.
The leaf extract of showed significant blood glucose reduction in the normoglycemic model and glucose loaded test at doses of 200 mg/kg (34.1%, < 0.001 and 55.5%, p < 0.001), respectively, as compared to the normal control. In the streptozotocin-induced diabetic model, extract and solvent fractions significantly (p < 0.001) reduced blood glucose level at all tested doses (100mg/kg, 200mg/kg and 400mg/kg) on the 14th day as compared to diabetic control. In addition, a significant reduction ( < 0.001) of serum TC, VLDL, LDL, TG was observed. In the antioxidant activity test, the IC values of extract and a standard drug (ascorbic acid) were 4.87µg/mL and 15.7µg/mL, respectively.
The present study showed that the methanolic leaf extract and solvent fractions of have antidiabetic, and antioxidant activity that provides a scientific support for the local use of the plant leaves in the treatment of diabetes.
目前，人们对来自天然治疗剂的新型抗糖尿病药物有需求，且糖尿病是一种全球性流行病。在埃塞俄比亚和厄立特里亚的传统医疗体系中，[植物名称]的叶子已被用于治疗糖尿病。本研究的目的是评估[植物名称]（瓦尔）叶子提取物及其溶剂萃取物在小鼠体内的抗糖尿病、降血脂作用以及体外抗氧化活性。
在正常血糖、葡萄糖负荷及链脲佐菌素诱导的糖尿病小鼠模型中，筛选[植物名称]叶子提取物及其溶剂萃取物的降血糖活性。在用叶子提取物及其溶剂萃取物治疗链脲佐菌素诱导的糖尿病小鼠时，测量体重和血脂水平。采用DPPH法测定植物叶子提取物的抗氧化活性。
与正常对照组相比，[植物名称]叶子提取物在正常血糖模型和葡萄糖负荷试验中，剂量为200mg/kg时分别显著降低血糖（34.1%，p<0.001和55.5%，p<0.001）。在链脲佐菌素诱导的糖尿病模型中，与糖尿病对照组相比，提取物及其溶剂萃取物在第14天所有测试剂量（100mg/kg、200mg/kg和400mg/kg）下均显著降低血糖水平（p<0.001）。此外，血清总胆固醇（TC）、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、甘油三酯（TG）显著降低（p<0.001）。在抗氧化活性试验中，提取物和标准药物（抗坏血酸）的半数抑制浓度（IC）值分别为4.87μg/mL和15.7μg/mL。
本研究表明，[植物名称]的甲醇叶提取物及其溶剂萃取物具有抗糖尿病和抗氧化活性，为该植物叶子在糖尿病治疗中的局部应用提供了科学依据。
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