Molla Mulugeta, Gemeda Negero, Abay Solomon M
School of Pharmacy, College of Health and Medical Sciences, Haramaya University, Haramaya, Ethiopia.
Traditional and Modern Medicine Research Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Evid Based Complement Alternat Med. 2017;2017:4103410. doi: 10.1155/2017/4103410. Epub 2017 May 9.
Fruits of A. DC. (Sapotaceae) are traditionally used for the treatment of diarrhea. The present study aimed at investigating modes of actions of this fruits for antidiarrheal action to guide future drug development process.
Fractions of chloroform, n-butanol, and water were obtained from 80% methanol extract, which was prepared by maceration. Antidiarrheal activities and the modes of actions were investigated in mice.
In castor oil induced diarrheal model, the extract delayed onset of diarrhea and reduced number and weight of feces at all tested doses significantly. In this model all fractions significantly delayed onset of diarrhea at all tested doses. Charcoal meal test showed that the extract and all the fractions produced a significant antimotility effect at all tested doses. Enteropooling test showed that the extract as well as n-butanol and aqueous fractions at all tested doses produced a significant decline in volume and weight of intestinal contents, whereas chloroform fraction had substantial effect only at high dose.
This study demonstrated that the extract and solvent fractions produced antidiarrheal activities due to dual inhibitory effect, intestinal motility, and fluid secretion, with the aqueous fraction being the most active among fractions in three models.
人心果科植物人心果的果实传统上用于治疗腹泻。本研究旨在探究该果实止泻作用的作用方式，以指导未来的药物开发过程。
通过浸渍法制备80%甲醇提取物，再从中获得氯仿、正丁醇和水部分。在小鼠中研究其止泻活性及作用方式。
在蓖麻油诱导的腹泻模型中，提取物在所有测试剂量下均显著延迟腹泻发作，并减少粪便数量和重量。在该模型中，所有部分在所有测试剂量下均显著延迟腹泻发作。炭末推进试验表明，提取物和所有部分在所有测试剂量下均产生显著的抗蠕动作用。肠积液试验表明，提取物以及正丁醇和水部分在所有测试剂量下均使肠内容物体积和重量显著下降，而氯仿部分仅在高剂量时有显著作用。
本研究表明，提取物和溶剂部分由于对肠道蠕动和液体分泌的双重抑制作用而产生止泻活性，其中水部分在三种模型中是各部分中活性最强的。
