Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Laboratory of Applied Periodontal and Peri-Implantitis Sciences, Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Clin Oral Investig. 2022 Feb;26(2):1773-1783. doi: 10.1007/s00784-021-04152-8. Epub 2021 Aug 30.
The aim of this study was to evaluate the in vitro effect of enamel matrix derivative (EMD) and hyaluronic acid (HA) and their synergistic combination on lipopolysaccharides (LPS)-induced inflammation in human keratinocytes and osteoblasts.
Cells were challenged with LPS (1 μg/ml) and cultured in the following treatment groups with EMD (30 mg/ml) and HA (30 mg/ml): LPS, EMD, HA, EMD + HA, EMD + LPS, HA + LPS, and EMD + HA + LPS. Cell viability, inflammatory cytokine expression, and cell migration were determined using colorimetric assay, quantitative real-time polymerase chain reaction (qPCR), and scratch wound healing assay, respectively.
Cell viability was decreased when exposed to LPS compared to the controls. Overall, LPS treatment expressed upregulation on inflammatory cytokine tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). EMD and HA reduced up to 3.0-fold the cytokine expression caused by LPS (p < 0.05). EMD and HA statistically induced higher migration in osteoblasts and keratinocytes, respectively. Migration was impaired by LPS, whereas it significantly increased after addition of EMD and HA.
EMD and HA are advantageous biomaterials that individually generate strong directional migratory keratinocyte and osteoblast response. Their combination also enhances cell viability, and anti-inflammatory and migratory abilities to promote healing specially under LPS inflammatory stimulus. Future in vivo and animal research is necessary to further characterize the effect of EMD and HA on periodontal regeneration.
The use of EMD in conjunction with HA resulted in a reduction of inflammation and improvement of tissue healing at wound sites. Both biomaterials combined may potentially improve the effectiveness of bone regeneration in periodontal bone defects, pointing to the potential clinical relevance of both materials in regenerative periodontal surgery.
本研究旨在评估釉基质衍生物（EMD）和透明质酸（HA）及其协同组合对人角质细胞和成骨细胞中脂多糖（LPS）诱导的炎症的体外影响。
用 LPS（1μg/ml）刺激细胞，并在以下处理组中用 EMD（30mg/ml）和 HA（30mg/ml）培养：LPS、EMD、HA、EMD+HA、EMD+LPS、HA+LPS 和 EMD+HA+LPS。使用比色法测定细胞活力、炎性细胞因子表达和细胞迁移，分别采用定量实时聚合酶链反应（qPCR）和划痕愈合试验。
与对照组相比，LPS 处理后细胞活力降低。总体而言，LPS 处理导致炎性细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素 1β（IL-1β）和白细胞介素 6（IL-6）表达上调。EMD 和 HA 将 LPS 引起的细胞因子表达降低了 3.0 倍（p<0.05）。EMD 和 HA 分别在成骨细胞和角质细胞中诱导更高的迁移，LPS 抑制迁移，但添加 EMD 和 HA 后迁移显著增加。
EMD 和 HA 是有利的生物材料，它们单独产生强烈的定向迁移角质细胞和成骨细胞反应。它们的组合还增强了细胞活力、抗炎和迁移能力，特别是在 LPS 炎症刺激下促进愈合。需要进行进一步的体内和动物研究，以进一步表征 EMD 和 HA 对牙周再生的影响。
EMD 与 HA 联合使用可减少炎症并改善伤口部位的组织愈合。两种生物材料联合使用可能会提高牙周骨缺损中骨再生的有效性，这表明这两种材料在再生性牙周手术中具有潜在的临床相关性。
