The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Microb Cell Fact. 2021 Aug 30;20(1):172. doi: 10.1186/s12934-021-01665-5.
L-valine is an essential amino acid that has wide and expanding applications with a suspected growing market demand. Its applicability ranges from animal feed additive, ingredient in cosmetic and special nutrients in pharmaceutical and agriculture fields. Currently, fermentation with the aid of model organisms, is a major method for the production of L-valine. However, achieving the optimal production has often been limited because of the metabolic imbalance in recombinant strains. In this review, the constrains in L-valine biosynthesis are discussed first. Then, we summarize the current advances in engineering of microbial cell factories that have been developed to address and overcome major challenges in the L-valine production process. Future prospects for enhancing the current L-valine production strategies are also discussed.
L-缬氨酸是一种必需氨基酸，具有广泛而不断扩大的应用，市场需求似乎也在不断增长。其应用范围从动物饲料添加剂、化妆品成分到制药和农业领域的特殊营养物质。目前，借助模式生物进行发酵是生产 L-缬氨酸的主要方法。然而，由于重组菌株中的代谢失衡，往往难以实现最佳生产。在这篇综述中，首先讨论了 L-缬氨酸生物合成的限制因素。然后，我们总结了目前在微生物细胞工厂工程方面的进展，这些进展旨在解决和克服 L-缬氨酸生产过程中的主要挑战。还讨论了增强当前 L-缬氨酸生产策略的未来前景。
