恒河猴中一种灭活的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）疫苗的免疫学评估。
Immunological evaluation of an inactivated SARS-CoV-2 vaccine in rhesus macaques.
作者信息
Chen Hongbo, Xie Zhongping, Long Runxiang, Fan Shengtao, Li Heng, He Zhanlong, Xu Kangwei, Liao Yun, Wang Lichun, Zhang Ying, Li Xueqi, Dong Xingqi, Mou Tangwei, Zhou Xiaofang, Yang Yaoyun, Guo Lei, Yang Jianbo, Zheng Huiwen, Xu Xingli, Li Jing, Liang Yan, Li Dandan, Zhao Zhimei, Hong Chao, Zhao Heng, Jiang Guorun, Che Yanchun, Yang Fengmei, Hu Yunguang, Wang Xi, Pu Jing, Ma Kaili, Wang Lin, Cheng Chen, Duan Weiguo, Shen Dong, Zhao Hongling, Jiang Ruiju, Deng Xinqiang, Li Yan, Zhu Hailian, Zhou Jian, Yu Li, Xu Mingjue, Yang Huijuan, Yi Li, Zhou Zhenxin, Yang Jiafang, Duan Nan, Yang Huan, Zhao Wangli, Yang Wei, Li Changgui, Liu Longding, Li Qihan
机构信息
Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming 650118, China.
National Institute of Food and Drug Control, Beijing 100050, China.
出版信息
Mol Ther Methods Clin Dev. 2021 Dec 10;23:108-118. doi: 10.1016/j.omtm.2021.08.005. Epub 2021 Aug 26.
Because of the relatively limited understanding of coronavirus disease 2019 (COVID-19) pathogenesis, immunological analysis for vaccine development is needed. Mice and macaques were immunized with an inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine prepared by two inactivators. Various immunological indexes were tested, and viral challenges were performed on day 7 or 150 after booster immunization in monkeys. This inactivated SARS-CoV-2 vaccine was produced by sequential inactivation with formaldehyde followed by propiolactone. The various antibody responses and specific T cell responses to different viral antigens elicited in immunized animals were maintained for longer than 150 days. This comprehensive immune response could effectively protect vaccinated macaques by inhibiting viral replication in macaques and substantially alleviating immunopathological damage, and no clinical manifestation of immunopathogenicity was observed in immunized individuals during viral challenge. This candidate inactivated vaccine was identified as being effective against SARS-CoV-2 challenge in rhesus macaques.
由于对2019冠状病毒病（COVID-19）发病机制的了解相对有限，因此需要进行用于疫苗研发的免疫学分析。用两种灭活剂制备的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）灭活疫苗对小鼠和猕猴进行免疫。检测了各种免疫学指标，并在加强免疫后第7天或第150天对猴子进行病毒攻击。这种灭活的SARS-CoV-2疫苗是通过先用甲醛然后用丙内酯进行顺序灭活生产的。免疫动物中引发的针对不同病毒抗原的各种抗体反应和特异性T细胞反应持续了150多天。这种全面的免疫反应可通过抑制猕猴体内的病毒复制并大幅减轻免疫病理损伤，有效保护接种疫苗的猕猴，并且在病毒攻击期间未在免疫个体中观察到免疫致病性的临床表现。这种候选灭活疫苗被确定对恒河猴的SARS-CoV-2攻击有效。
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