Hsieh Chen-Che, Yi Tsung-Kai, Kao Yi-Feng, Lin Shin-Ping, Tu Ming-Chieh, Chou Yu-Chieh, Lu Jheng-Jhe, Chai Huey-Jine, Cheng Kuan-Chen
Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 81157, Taiwan.
Seafood Technology Division, Fisheries Research Institute, Ministry of Agriculture, Keelung 20246, Taiwan.
Molecules. 2024 Nov 30;29(23):5688. doi: 10.3390/molecules29235688.
is a red macroalga known for its bioactive compounds with antioxidant, anti-inflammatory, and skin-regenerative properties. The study aimed to examine their effects on UV protection, collagen synthesis, fibroblast proliferation, and pigmentation modulation. Bioactive compounds were extracted using two solvents, producing ethanol extract (FE) and alkaline extracts (AE). Methods involved characterizing extracts using mass spectrometry and assessing their effects on human fibroblasts under UVB-induced damage. UV absorbance, ROS production, and collagen synthesis were evaluated. The FE extract, which comprised 4-hydroxyquinoline, phytosphingosine, and docosapentaenoic acid, reinstated procollagen type I synthesis to 113% of baseline levels and reduced TGF-β1-mediated fibroblast proliferation to 87.78%. FE also suppressed and by 71% and 68%, respectively, indicating modulation of fibrosis-associated pathways. AE, containing 4-hydroxyquinoline and phenylalanine betaine, demonstrated dose-responsive cellular repair, reducing fibroblast proliferation to 97.86% and Type I expression by 73% at 1000 μg/mL. Both extracts decreased ROS production, with FE and AE reducing levels by 21.4% and 19.7%, respectively, under UVB-induced oxidative stress. FE showed superior scar inhibition, while AE excelled in skin regeneration and pigmentation management.
是一种红色大型海藻，以其具有抗氧化、抗炎和皮肤再生特性的生物活性化合物而闻名。该研究旨在考察它们对紫外线防护、胶原蛋白合成、成纤维细胞增殖和色素沉着调节的影响。使用两种溶剂提取生物活性化合物，得到乙醇提取物（FE）和碱性提取物（AE）。方法包括用质谱对提取物进行表征，并评估它们对紫外线B诱导损伤下的人成纤维细胞的影响。评估了紫外线吸光度、活性氧生成和胶原蛋白合成。FE提取物含有4-羟基喹啉、植物鞘氨醇和二十二碳五烯酸，将I型前胶原蛋白合成恢复至基线水平的113%，并将转化生长因子-β1介导的成纤维细胞增殖降低至87.78%。FE还分别抑制 和 达71%和68%，表明对纤维化相关途径有调节作用。AE含有4-羟基喹啉和苯丙氨酸甜菜碱，表现出剂量依赖性的细胞修复作用，在1000μg/mL时将成纤维细胞增殖降低至97.86%，并将I型 表达降低73%。两种提取物均降低了活性氧的生成，在紫外线B诱导的氧化应激下，FE和AE分别将活性氧水平降低了21.4%和19.7%。FE在瘢痕抑制方面表现优异，而AE在皮肤再生和色素沉着管理方面表现出色。
